The hematological response to exhaustive exercise in 'all-fish' growth hormone transgenic common carp (Cyprinus carpio L.)
文献类型：期刊论文
| 作者 | Li, Deliang1,2; Fu, Cuizhang1,3; Wang, Yaping1; Zhu, Zuoyan1; Hu, Wei1 |
| 刊名 | AQUACULTURE
 |
| 出版日期 | 2011-02-03 |
| 卷号 | 311期号:1-4页码:263-268 |
| 关键词 | Common carp Transgenic Exhaustive exercise Growth hormone (GH) Hematology |
| ISSN号 | 0044-8486 |
| 通讯作者 | Fu, CZ, Wuhan Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; czfu@fudan.edu.cn, huwei@ihb.ac.cn |
| 中文摘要 | This study examined the hematological profiles and responses to exhaustive exercise in 'all-fish' growth hormone (GH) transgenic common carp (Cyprinus carpio L) compared to non-transgenic controls. Transgenic fish had significantly smaller erythrocytes (length, width, surface area and volume), with a larger surface to volume ratios than non-transgenics. There were no significant differences in hematocrit, blood hemoglobin concentrations, plasma glucose, lactate, cholesterol and protein concentrations between the two strains at rest, but the transgenic fish had significantly lower resting mean corpuscular hemoglobin concentrations (MCHC) or higher plasma triglyceride concentrations than controls. Exhaustive exercise did not induce significant changes in hematocrit, hemoglobin concentrations and MCHC in either strain. The patterns of elevation and recovery in plasma glucose and lactate were the same for transgenic and control fish, although lactate levels were lower in the transgnic fish than controls at 1 h and 2 h post-exercise. There was a significant elevation in plasma cholesterol and triglyceride concentrations for 1 h post-exercise in transgenics but not in non-transgenic controls. Plasma protein concentrations were not altered by exhaustive exercise in transgenics but were lower from 2 h post-exercise in non-transgenic controls. These findings suggest that the 'all-fish' GH transgene significantly affects the erythrocyte morphology and the patterns in hematological response to exhaustive exercise of common carp, especially for lipid metabolism. The greater reliance on triglycerides and a greater erythrocyte surface to volume ratio may help transgenic carp to recover from exhaustive exercise, but further research on muscle metabolism and aerobic metabolic rate are needed in order to confirm this conclusion. (C) 2010 Elsevier B.V. All rights reserved. |
| 英文摘要 | This study examined the hematological profiles and responses to exhaustive exercise in 'all-fish' growth hormone (GH) transgenic common carp (Cyprinus carpio L) compared to non-transgenic controls. Transgenic fish had significantly smaller erythrocytes (length, width, surface area and volume), with a larger surface to volume ratios than non-transgenics. There were no significant differences in hematocrit, blood hemoglobin concentrations, plasma glucose, lactate, cholesterol and protein concentrations between the two strains at rest, but the transgenic fish had significantly lower resting mean corpuscular hemoglobin concentrations (MCHC) or higher plasma triglyceride concentrations than controls. Exhaustive exercise did not induce significant changes in hematocrit, hemoglobin concentrations and MCHC in either strain. The patterns of elevation and recovery in plasma glucose and lactate were the same for transgenic and control fish, although lactate levels were lower in the transgnic fish than controls at 1 h and 2 h post-exercise. There was a significant elevation in plasma cholesterol and triglyceride concentrations for 1 h post-exercise in transgenics but not in non-transgenic controls. Plasma protein concentrations were not altered by exhaustive exercise in transgenics but were lower from 2 h post-exercise in non-transgenic controls. These findings suggest that the 'all-fish' GH transgene significantly affects the erythrocyte morphology and the patterns in hematological response to exhaustive exercise of common carp, especially for lipid metabolism. The greater reliance on triglycerides and a greater erythrocyte surface to volume ratio may help transgenic carp to recover from exhaustive exercise, but further research on muscle metabolism and aerobic metabolic rate are needed in order to confirm this conclusion. (C) 2010 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Fisheries; Marine & Freshwater Biology |
| 类目[WOS] | Fisheries ; Marine & Freshwater Biology |
| 研究领域[WOS] | Fisheries ; Marine & Freshwater Biology |
| 关键词[WOS] | RAINBOW-TROUT ; WHITE MUSCLE ; ACID-BASE ; METABOLISM ; STRESS ; CORTISOL ; RECOVERY ; BLOOD ; STRAINS ; VOLUME |
| 收录类别 | SCI |
| 资助信息 | Development Plan of the State Key Fundamental Research of China [2007CB109205, 2009CB118804]; National Natural Science Foundation [30930069] |
| 语种 | 英语 |
| WOS记录号 | WOS:000287432600039 |
| 公开日期 | 2011-04-14 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/15488]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Wuhan Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China 3.Fudan Univ, Inst Biodivers Sci, Key Lab Biodivers Sci & Ecol Engn, Minist Educ, Shanghai 200433, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Deliang,Fu, Cuizhang,Wang, Yaping,et al. The hematological response to exhaustive exercise in 'all-fish' growth hormone transgenic common carp (Cyprinus carpio L.)[J]. AQUACULTURE,2011,311(1-4):263-268. |
| APA | Li, Deliang,Fu, Cuizhang,Wang, Yaping,Zhu, Zuoyan,&Hu, Wei.(2011).The hematological response to exhaustive exercise in 'all-fish' growth hormone transgenic common carp (Cyprinus carpio L.).AQUACULTURE,311(1-4),263-268. |
| MLA | Li, Deliang,et al."The hematological response to exhaustive exercise in 'all-fish' growth hormone transgenic common carp (Cyprinus carpio L.)".AQUACULTURE 311.1-4(2011):263-268. |
入库方式： OAI收割
来源：水生生物研究所
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