Genome of turbot rhabdovirus exhibits unusual non-coding regions and an additional ORF that could be expressed in fish cell
文献类型：期刊论文
| 作者 | Zhu, Ruo-Lin; Lei, Xiao-Ying; Ke, Fei; Yuan, Xiu-Ping; Zhang, Qi-Ya |
| 刊名 | VIRUS RESEARCH
 |
| 出版日期 | 2011-02-01 |
| 卷号 | 155期号:2页码:495-505 |
| 关键词 | Fish rhabdovirus Genome Turbot Scophthalmus maximus Non-coding region Expressed ORF Ps |
| ISSN号 | 0168-1702 |
| 通讯作者 | Zhang, QY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Grad Sch, Wuhan 430072, Peoples R China ; zhangqy@ihb.ac.cn |
| 中文摘要 | Genomic sequence of Scophthalmus maximus rhabdovirus (SMRV) isolated from diseased turbot has been characterized. The complete genome of SMRV comprises 11,492 nucleotides and encodes five typical rhabdovirus genes N, P. M, G and L In addition, two open reading frames (ORF) are predicted overlapping with P gene, one upstream of P and smaller than P (temporarily called Ps), and another in P gene which may encodes a protein similar to the vesicular stomatitis virus C protein. The C ORF is contained within the P ORF. The five typical proteins share the highest sequence identities (48.9%) with the corresponding proteins of rhabdoviruses in genus Vesiculovirus. Phylogenetic analysis of partial L protein sequence indicates that SMRV is close to genus Vesiculovirus. The first 13 nucleotides at the ends of the SMRV genome are absolutely inverse complementarity. The gene junctions between the five genes show conserved polyadenylation signal (CATGA(7)) and intergenic dinucleotide (CT) followed by putative transcription initiation sequence A(A/G)(C/G)A(A/G/T), which are different from known rhabdoviruses. The entire Ps ORF was cloned and expressed, and used to generate polyclonal antibody in mice. One obvious band could be detected in SMRV-infected carp leucocyte cells (CLCs) by anti-Ps/C serum via Western blot, and the subcellular localization of Ps-GFP fusion protein exhibited cytoplasm distribution as multiple punctuate or doughnut shaped foci of uneven size. (C) 2010 Elsevier B.V. All rights reserved. |
| 英文摘要 | Genomic sequence of Scophthalmus maximus rhabdovirus (SMRV) isolated from diseased turbot has been characterized. The complete genome of SMRV comprises 11,492 nucleotides and encodes five typical rhabdovirus genes N, P. M, G and L In addition, two open reading frames (ORF) are predicted overlapping with P gene, one upstream of P and smaller than P (temporarily called Ps), and another in P gene which may encodes a protein similar to the vesicular stomatitis virus C protein. The C ORF is contained within the P ORF. The five typical proteins share the highest sequence identities (48.9%) with the corresponding proteins of rhabdoviruses in genus Vesiculovirus. Phylogenetic analysis of partial L protein sequence indicates that SMRV is close to genus Vesiculovirus. The first 13 nucleotides at the ends of the SMRV genome are absolutely inverse complementarity. The gene junctions between the five genes show conserved polyadenylation signal (CATGA(7)) and intergenic dinucleotide (CT) followed by putative transcription initiation sequence A(A/G)(C/G)A(A/G/T), which are different from known rhabdoviruses. The entire Ps ORF was cloned and expressed, and used to generate polyclonal antibody in mice. One obvious band could be detected in SMRV-infected carp leucocyte cells (CLCs) by anti-Ps/C serum via Western blot, and the subcellular localization of Ps-GFP fusion protein exhibited cytoplasm distribution as multiple punctuate or doughnut shaped foci of uneven size. (C) 2010 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Virology |
| 类目[WOS] | Virology |
| 研究领域[WOS] | Virology |
| 关键词[WOS] | VESICULAR STOMATITIS-VIRUS ; RANA-GRYLIO-VIRUS ; SCOPHTHALMUS-MAXIMUS RHABDOVIRUS ; HEMATOPOIETIC NECROSIS VIRUS ; MONOCLONAL-ANTIBODIES ; SPRING VIREMIA ; ENVELOPE PROTEIN ; RNA-POLYMERASE ; HOST PROTEINS ; MANDARIN FISH |
| 收录类别 | SCI |
| 资助信息 | National Major Basic Research Program [2009CB118704, 2010CB126303]; National 863 High Technology Research Foundation of China [2006AA10A402]; National Natural Science Foundation of China [30871938, 31072239]; Agriculture Commonweal Scientific Research Plan [200803013]; Chinese Academy of Sciences [KSCX2-YW-G-075-19] |
| 语种 | 英语 |
| WOS记录号 | WOS:000287544400014 |
| 公开日期 | 2011-04-14 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/15490]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Grad Sch, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Ruo-Lin,Lei, Xiao-Ying,Ke, Fei,et al. Genome of turbot rhabdovirus exhibits unusual non-coding regions and an additional ORF that could be expressed in fish cell[J]. VIRUS RESEARCH,2011,155(2):495-505. |
| APA | Zhu, Ruo-Lin,Lei, Xiao-Ying,Ke, Fei,Yuan, Xiu-Ping,&Zhang, Qi-Ya.(2011).Genome of turbot rhabdovirus exhibits unusual non-coding regions and an additional ORF that could be expressed in fish cell.VIRUS RESEARCH,155(2),495-505. |
| MLA | Zhu, Ruo-Lin,et al."Genome of turbot rhabdovirus exhibits unusual non-coding regions and an additional ORF that could be expressed in fish cell".VIRUS RESEARCH 155.2(2011):495-505. |
入库方式： OAI收割
来源：水生生物研究所
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