Critical Developmental Stages for the Efficiency of Somatic Cell Nuclear Transfer in Zebrafish
文献类型：期刊论文
| 作者 | Luo, Da-Ji1,2; Hu, Wei1; Chen, Shang-Ping1; Zhu, Zuo-Yan1 |
| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
 |
| 出版日期 | 2011 |
| 卷号 | 7期号:4页码:476-486 |
| 关键词 | SCNT reprogramming dome stage shield stage klf4 and mycb |
| ISSN号 | 1449-2288 |
| 通讯作者 | Hu, W, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; huwei@ihb.ac.cn |
| 中文摘要 | Somatic cell nuclear transfer (SCNT) has been performed extensively in fish since the 1960s with a generally low efficiency of approximately 1%. Little is known about somatic nuclear reprogramming in fish. Here, we utilized the zebrafish as a model to study reprogramming events of nuclei from tail, liver and kidney cells by SCNT. We produced a total of 4,796 reconstituted embryos and obtained a high survival rate of 58.9-67.4% initially at the 8-cell stage. The survival rate exhibited two steps of dramatic decrease, leading to 8.7-13.9% at the dome stage and to 1.5-2.96% by the shield stage. Concurrently, we observed that SCNT embryos displayed apparently delayed development also at the two stages, namely the dome stage (1:30 +/- 0:40) and the shield stage (2:50 +/- 0:50), indicating that the dome and shield stage are critical for the SCNT efficiency. Interestingly, we also revealed that an apparent alteration in klf4 and mycb expression occurred at the dome stage in SCNT embryos from all the three donor cell sources. Taken together, these results suggest that the dome stage is critical for the SCNT efficiency, and that alternated gene expression appears to be common to SCNT embryos independently of the donor cell types, suggesting that balanced mycb and klf4 expression at this stage is important for proper reprogramming of somatic nuclei in zebrafish SCNT embryos. Although the significant alteration in klf4 and mycb expression was not identified at the shield stage between ZD and SCNT embryos, the importance of reprogramming processes at the shield stage should not be underestimated in zebrafish SCNT embryos. |
| 英文摘要 | Somatic cell nuclear transfer (SCNT) has been performed extensively in fish since the 1960s with a generally low efficiency of approximately 1%. Little is known about somatic nuclear reprogramming in fish. Here, we utilized the zebrafish as a model to study reprogramming events of nuclei from tail, liver and kidney cells by SCNT. We produced a total of 4,796 reconstituted embryos and obtained a high survival rate of 58.9-67.4% initially at the 8-cell stage. The survival rate exhibited two steps of dramatic decrease, leading to 8.7-13.9% at the dome stage and to 1.5-2.96% by the shield stage. Concurrently, we observed that SCNT embryos displayed apparently delayed development also at the two stages, namely the dome stage (1:30 +/- 0:40) and the shield stage (2:50 +/- 0:50), indicating that the dome and shield stage are critical for the SCNT efficiency. Interestingly, we also revealed that an apparent alteration in klf4 and mycb expression occurred at the dome stage in SCNT embryos from all the three donor cell sources. Taken together, these results suggest that the dome stage is critical for the SCNT efficiency, and that alternated gene expression appears to be common to SCNT embryos independently of the donor cell types, suggesting that balanced mycb and klf4 expression at this stage is important for proper reprogramming of somatic nuclei in zebrafish SCNT embryos. Although the significant alteration in klf4 and mycb expression was not identified at the shield stage between ZD and SCNT embryos, the importance of reprogramming processes at the shield stage should not be underestimated in zebrafish SCNT embryos. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biochemistry & Molecular Biology; Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics |
| 关键词[WOS] | DIFFERENTIALLY EXPRESSED GENES ; MEDAKA ORYZIAS-LATIPES ; ENUCLEATED EGGS ; STEM-CELLS ; CLONED EMBRYOS ; FISH ; CLONING ; TRANSPLANTATION ; MICE ; IDENTIFICATION |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [30900853]; Specialized Research Fund for the Doctoral Program of Higher Education of China [20090141120015]; China Postdoctoral Science Foundation [201003505] |
| 语种 | 英语 |
| WOS记录号 | WOS:000289849400011 |
| 公开日期 | 2011-06-30 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/15622]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Wuhan Univ, Sch Basic Med Sci, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Luo, Da-Ji,Hu, Wei,Chen, Shang-Ping,et al. Critical Developmental Stages for the Efficiency of Somatic Cell Nuclear Transfer in Zebrafish[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,2011,7(4):476-486. |
| APA | Luo, Da-Ji,Hu, Wei,Chen, Shang-Ping,&Zhu, Zuo-Yan.(2011).Critical Developmental Stages for the Efficiency of Somatic Cell Nuclear Transfer in Zebrafish.INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,7(4),476-486. |
| MLA | Luo, Da-Ji,et al."Critical Developmental Stages for the Efficiency of Somatic Cell Nuclear Transfer in Zebrafish".INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES 7.4(2011):476-486. |
入库方式： OAI收割
来源：水生生物研究所
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