Characterization of two C-type lectin-like domain (CTLD)-containing proteins from the cDNA library of Chinese mitten crab Eriocheir sinensis
文献类型：期刊论文
| 作者 | Guo, H. Z.1,2,3; Zou, P. F.1,2; Fu, J. P.1,2; Guo, Z.1,2; Zhu, B. K.3; Nie, P.1,2; Chang, M. X.1,2 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2011-02-01 |
| 卷号 | 30期号:2页码:515-524 |
| 关键词 | Eriocheir sinensis cDNA library C-type lectin-like domain (CTLD)-containing proteins Expression |
| ISSN号 | 1050-4648 |
| 通讯作者 | Chang, MX, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China ; mingxianchang@ihb.ac.cn |
| 中文摘要 | Chinese mitten crab Eriocheir sinensis is one of the most important aquaculture crustacean species in China. A cDNA library was constructed from mixed tissues of E. sinensis challenged with LPS. Eight genes involved in immune response were identified from 319 single colonies. Among them, two different C-type lectin-like domain (CTLD)-containing proteins were firstly identified in Chinese mitten crab. The full-length cDNA sequences of two C-type lectin-like domain (CTLD)-containing proteins named E5CTLDcp-1 and EsCTLDcp-2 were cloned by 5' RACE. The deduced amino acid sequences of E5CTLDcp-1 and E5CTLDcp-2 possessed several conserved features of C-type lectin subfamily. The tissue distribution of EsCTLDcp-1 and EsCTLDcp-2 was examined by Real-time PCR. In the normal Chinese mitten crab, the expression of E5CTLDcp-2 was detected in all tested tissues such as haemolymph, muscle, intestine, gill, heart, gonad and hepatopancreas, whereas in muscle, intestine, gill, heart and hepatopancreas for EsCTLDcp-1. The highest expressions of EsCTLDcp-1 and E5CliDcp-2 were both observed in hepatopancreas. LPS significantly induced the expression of EsCTLDcp-1 and EsCTLDcp-2 in the hepatopancreas at the different time points. The induced fold change of E5CTLDcp-1 and EsCTLDcp-2 increased significantly from 2 h for EsCTLDcp-1 and 4 h for E5CTLDcp-2, and reached a maximum at 12 h, then dropped at 24 h. A differential pattern was found in Chinese mitten crab challenged with Chinese mitten crab pathogen Aeromonas hydrophila. The expression of EsCTLDcp-1 increased significantly at 2 h post-challenge crabs with A. hydrophila, then decreased at 4 h and 8 h, after that increased at 12 h and 24 h. The expression of EsCTLDcp-2 was decreased at the all time points. All these data suggest a differential role of E5CILDcp-1 and EsCTLDcp-2 in the crab innate immune response to bacterial infection. (C) 2010 Elsevier Ltd. All rights reserved. |
| 英文摘要 | Chinese mitten crab Eriocheir sinensis is one of the most important aquaculture crustacean species in China. A cDNA library was constructed from mixed tissues of E. sinensis challenged with LPS. Eight genes involved in immune response were identified from 319 single colonies. Among them, two different C-type lectin-like domain (CTLD)-containing proteins were firstly identified in Chinese mitten crab. The full-length cDNA sequences of two C-type lectin-like domain (CTLD)-containing proteins named E5CTLDcp-1 and EsCTLDcp-2 were cloned by 5' RACE. The deduced amino acid sequences of E5CTLDcp-1 and E5CTLDcp-2 possessed several conserved features of C-type lectin subfamily. The tissue distribution of EsCTLDcp-1 and EsCTLDcp-2 was examined by Real-time PCR. In the normal Chinese mitten crab, the expression of E5CTLDcp-2 was detected in all tested tissues such as haemolymph, muscle, intestine, gill, heart, gonad and hepatopancreas, whereas in muscle, intestine, gill, heart and hepatopancreas for EsCTLDcp-1. The highest expressions of EsCTLDcp-1 and E5CliDcp-2 were both observed in hepatopancreas. LPS significantly induced the expression of EsCTLDcp-1 and EsCTLDcp-2 in the hepatopancreas at the different time points. The induced fold change of E5CTLDcp-1 and EsCTLDcp-2 increased significantly from 2 h for EsCTLDcp-1 and 4 h for E5CTLDcp-2, and reached a maximum at 12 h, then dropped at 24 h. A differential pattern was found in Chinese mitten crab challenged with Chinese mitten crab pathogen Aeromonas hydrophila. The expression of EsCTLDcp-1 increased significantly at 2 h post-challenge crabs with A. hydrophila, then decreased at 4 h and 8 h, after that increased at 12 h and 24 h. The expression of EsCTLDcp-2 was decreased at the all time points. All these data suggest a differential role of E5CILDcp-1 and EsCTLDcp-2 in the crab innate immune response to bacterial infection. (C) 2010 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences |
| 类目[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 关键词[WOS] | SHRIMP FENNEROPENAEUS-CHINENSIS ; MOLECULAR-CLONING ; INNATE IMMUNITY ; ANTILIPOPOLYSACCHARIDE FACTOR ; CAENORHABDITIS-ELEGANS ; ANTIMICROBIAL ACTIVITY ; TREMOR DISEASE ; RECOGNITION ; HEPATOPANCREAS ; SEQUENCE |
| 收录类别 | SCI |
| 资助信息 | National Key Technology RD Program [2007BAD37B03]; National Natural Science Foundation of China [30830083] |
| 语种 | 英语 |
| WOS记录号 | WOS:000288305300010 |
| 公开日期 | 2011-06-30 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/15632]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Lab Fish Dis, Wuhan 430072, Hubei Province, Peoples R China 3.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Hubei Province, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, H. Z.,Zou, P. F.,Fu, J. P.,et al. Characterization of two C-type lectin-like domain (CTLD)-containing proteins from the cDNA library of Chinese mitten crab Eriocheir sinensis[J]. FISH & SHELLFISH IMMUNOLOGY,2011,30(2):515-524. |
| APA | Guo, H. Z..,Zou, P. F..,Fu, J. P..,Guo, Z..,Zhu, B. K..,...&Chang, M. X..(2011).Characterization of two C-type lectin-like domain (CTLD)-containing proteins from the cDNA library of Chinese mitten crab Eriocheir sinensis.FISH & SHELLFISH IMMUNOLOGY,30(2),515-524. |
| MLA | Guo, H. Z.,et al."Characterization of two C-type lectin-like domain (CTLD)-containing proteins from the cDNA library of Chinese mitten crab Eriocheir sinensis".FISH & SHELLFISH IMMUNOLOGY 30.2(2011):515-524. |
入库方式： OAI收割
来源：水生生物研究所
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