Production, characterization, and applications of mouse monoclonal antibodies against gonadotropin, somatolactin, and prolactin from common carp (Cyprinus carpio)
文献类型：会议论文
| 作者 | Xu, Zhuwei ; Li, Qi ; Wang, Yun ; Song, Chaojun ; Zhang, Tao ; Chen, Lihua ; Ji, Jianguo ; Yang, Angang ; Zhu, Zuoyan ; Hu, Wei ; Jin, Boquan |
| 出版日期 | 2010 |
| 会议名称 | 2nd International Symposium for Fish Growth and Reporduction |
| 会议日期 | JUN 20-21, 2009 |
| 会议地点 | Hong Kong, PEOPLES R CHINA |
| 关键词 | Common carp ELISA Gonadotropin Monoclonal antibody Prolactin Somatolactin |
| 页码 | 373-378 |
| 通讯作者 | Hu, W, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 中文摘要 | The gonadotropin alpha subunit (cGTH alpha), gonadotropinII beta subunit (cGTHII beta), somatolactin (cSL), and prolactin (cPRL) were isolated from the pituitaries of common carps, purified by traditional chromatographic analysis, identified by mass-chromatographic analysis, and used as immunogens in the B-lymphocyte hybridoma technique. Totally, 7, 11, 17, and 8 hybridoma cell lines were established, which were able to stably secrete monoclonal antibodies (mAbs) against cGTH alpha, cGTHII beta, cSL, and cPRL, and designated as FMU-cGTH alpha 1-7, FMU-cGTHII beta 1-11, FMU-cSL 1-17, and FMU-cPRL 1-8, respectively. The isotype, titer, and specificity were identified by enzyme-linked immunosorbent assay (ELISA), Western blot, and immunohistochemical staining, respectively, and application of these mAbs in the aforementioned tests has been proved. Furthermore, sensitive sandwich-ELISA systems for quantitative detection of the hormones mentioned above were also developed. (C) 2009 Elsevier Inc. All rights reserved. |
| 收录类别 | SCI |
| 会议录 | GENERAL AND COMPARATIVE ENDOCRINOLOGY
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| 会议录出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 会议录出版地 | SAN DIEGO |
| 语种 | 英语 |
| ISSN号 | 0016-6480 |
| WOS记录号 | WOS:000278875100007 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/13874]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_会议论文 |
| 推荐引用方式 GB/T 7714 | Xu, Zhuwei,Li, Qi,Wang, Yun,et al. Production, characterization, and applications of mouse monoclonal antibodies against gonadotropin, somatolactin, and prolactin from common carp (Cyprinus carpio)[C]. 见:2nd International Symposium for Fish Growth and Reporduction. Hong Kong, PEOPLES R CHINA. JUN 20-21, 2009. |
入库方式： OAI收割
来源：水生生物研究所
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