A Label-Free Protein Microfluidic Array for Parallel Immunoassays
文献类型：期刊论文
| 作者 | Wang ZH(王战会); Meng YH(孟永宏) ; Ying PQ(应佩青); Qi C(齐财); Jin G(靳刚)
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| 刊名 | Electrophoresis
 |
| 出版日期 | 2006 |
| 卷号 | 27期号:20页码:4078-4085 |
| 通讯作者邮箱 | gajin@imech.ac.cn |
| ISSN号 | 0173-0835 |
| 通讯作者 | Jin, G (reprint author), Chinese Acad Sci, Inst Mech, Beijing 100080, Peoples R China. |
| 中文摘要 | A label-free protein microfluidic array for immunoassays based on the combination of imaging ellipsometry and an integrated microfluidic system is presented. Proteins can be patterned homogeneously on substrate in array format by the microfluidic system simultaneously. After preparation, the protein array can be packed in the microfluidic system which is full of buffer so that proteins are not exposed to denaturing conditions. With simple microfluidic channel junction, the protein microfluidic array can be used in serial or parallel format to analyze single or multiple samples simultaneously. Imaging ellipsometry is used for the protein array reading with a label-free format. The biological and medical applications of the label-free protein microfluidic array are demonstrated by screening for antibody–antigen interactions, measuring the concentration of the protein solution and detecting five markers of hepatitis B. |
| 学科主题 | 力学 |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | IMAGING ELLIPSOMETRY ; HIGH-SENSITIVITY ; MICROARRAYS ; SYSTEMS ; ASSAY ; VISUALIZATION ; CHALLENGES ; BIOSENSOR ; SURFACES ; ANTIBODY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000241916100020 |
| 公开日期 | 2007-06-15 ; 2007-12-05 ; 2009-06-23 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/17518]  |
| 专题 | 力学研究所_力学所知识产出(1956-2008) |
| 推荐引用方式 GB/T 7714 | Wang ZH,Meng YH,Ying PQ,et al. A Label-Free Protein Microfluidic Array for Parallel Immunoassays[J]. Electrophoresis,2006,27(20):4078-4085. |
| APA | 王战会,孟永宏,应佩青,齐财,&靳刚.(2006).A Label-Free Protein Microfluidic Array for Parallel Immunoassays.Electrophoresis,27(20),4078-4085. |
| MLA | 王战会,et al."A Label-Free Protein Microfluidic Array for Parallel Immunoassays".Electrophoresis 27.20(2006):4078-4085. |
入库方式： OAI收割
来源：力学研究所
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