Determination of vitamin B-1 in seawater and microalgal fermentation media by high-performance liquid chromatography with fluorescence detection
文献类型：期刊论文
| 作者 | He, HZ ; Li, HB ; Chen, F |
| 刊名 | ANALYTICAL AND BIOANALYTICAL CHEMISTRY
 |
| 出版日期 | 2005 |
| 卷号 | 383期号:5页码:875-879 |
| 关键词 | vitamin B-1 high-performance liquid chromatography solid-phase extraction fermentation media seawater |
| ISSN号 | 1618-2642 |
| 通讯作者 | sfchen@hkusua.hku.hk |
| 中文摘要 | A highly sensitive high-performance liquid chromatographic method with fluorescence detection has been developed for determination of vitamin B-1. Vitamin B-1 was converted into a fluorescent compound by treatment with hydrogen peroxide-horseradish peroxidase and the derivative was subsequently analyzed by HPLC on a Waters Spherisorb ODS2 column (250 mmx4.6 mm ID, 5 mu m) with 40:60 methanol-pH 8.5 acetate buffer solution as mobile phase and fluorescence detection at 440 nm (with excitation at 375 nm). The calibration graph was linear from 5.00x10(-10) mol L-1 to 5.00x10(-7) mol L-1 for vitamin B-1 with a correlation coefficient of 0.9991 (n=9). The detection limit was 1.0x10(-10) mol L-1. The method was successfully used for determination of vitamin B-1 at pg mL(-1) levels in microalgal fermentation media and seawater after solid-phase extraction. Recovery was from 89 to 110% and the relative standard deviation was in the range 1.1 to 4.3%. |
| 学科主题 | Biochemical Research Methods; Chemistry, Analytical |
| 收录类别 | SCI |
| WOS记录号 | WOS:000233874900026 |
| 公开日期 | 2011-07-03 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/5683]  |
| 专题 | 南海海洋研究所_热带海洋环境国家重点实验室(LTO) |
| 推荐引用方式 GB/T 7714 | He, HZ,Li, HB,Chen, F. Determination of vitamin B-1 in seawater and microalgal fermentation media by high-performance liquid chromatography with fluorescence detection[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2005,383(5):875-879. |
| APA | He, HZ,Li, HB,&Chen, F.(2005).Determination of vitamin B-1 in seawater and microalgal fermentation media by high-performance liquid chromatography with fluorescence detection.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,383(5),875-879. |
| MLA | He, HZ,et al."Determination of vitamin B-1 in seawater and microalgal fermentation media by high-performance liquid chromatography with fluorescence detection".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 383.5(2005):875-879. |
入库方式： OAI收割
来源：南海海洋研究所
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