Determination of paralytic shellfish poisoning toxins in cultured microalgae by high-performance liquid chromatography with fluorescence detection
文献类型：期刊论文
| 作者 | He, HZ ; Li, HB ; Jiang, Y ; Chen, F |
| 刊名 | ANALYTICAL AND BIOANALYTICAL CHEMISTRY
 |
| 出版日期 | 2005 |
| 卷号 | 383期号:6页码:1014-1017 |
| 关键词 | paralytic shellfish poisoning toxins high performance liquid chromatography fluorescence detection microalgae |
| ISSN号 | 1618-2642 |
| 通讯作者 | sfchen@hkusua.hku.hk |
| 中文摘要 | A novel method for the determination of paralytic shellfish poisoning (PSP) toxins using high-performance liquid chromatography with fluorescence detection was developed. The fluorescent derivates of neosaxitoxin (neoSTX), saxitoxin (STX), gonyautoxins 1 and 4 (GTX1+4), and gonyautoxins 2 and 3 (GTX2+3) were separated on a mu Bondapak NH2 column (300 mm x 3.9 mm, 10 mu m) using water and acetate buffer (pH 6.5) as the mobile phase (1.00 mL min(-1)) in gradient mode with fluorescence detection at 390 nm (excitation at 330 nm). The linear ranges of neoSTX, STX, GTX1+4 and GTX2+3 were 3.31-331, 0.952-95.2, 3.78-378 and 0.124-12.4 ng mL(-1), respectively. The detection limits of neoSTX, STX, GTX1+4 and GTX2+3 were 1.10, 0.32, 1.26 and 0.041 ng mL(-1), respectively. The method was successfully applied to the determination of PSP toxins in microalgae. The recoveries ranged from 88 +/- 2% to 107 +/- 4% and the relative standard deviations were 0.16% to 4.4%. The procedure is also environmentally friendly because no organic solvent is used in the mobile phase. |
| 学科主题 | Biochemical Research Methods; Chemistry, Analytical |
| 收录类别 | SCI |
| WOS记录号 | WOS:000233875200017 |
| 公开日期 | 2011-07-03 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/5685]  |
| 专题 | 南海海洋研究所_热带海洋环境国家重点实验室(LTO) |
| 推荐引用方式 GB/T 7714 | He, HZ,Li, HB,Jiang, Y,et al. Determination of paralytic shellfish poisoning toxins in cultured microalgae by high-performance liquid chromatography with fluorescence detection[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2005,383(6):1014-1017. |
| APA | He, HZ,Li, HB,Jiang, Y,&Chen, F.(2005).Determination of paralytic shellfish poisoning toxins in cultured microalgae by high-performance liquid chromatography with fluorescence detection.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,383(6),1014-1017. |
| MLA | He, HZ,et al."Determination of paralytic shellfish poisoning toxins in cultured microalgae by high-performance liquid chromatography with fluorescence detection".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 383.6(2005):1014-1017. |
入库方式： OAI收割
来源：南海海洋研究所
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