Biodegradation of 1-methylindole and 3-methylindole by mangrove sediment enrichment cultures and a pure culture of an isolated Pseudomonas aeruginosa Gs
文献类型：期刊论文
| 作者 | Yin, B ; Huang, LM ; Gu, JD |
| 刊名 | WATER AIR AND SOIL POLLUTION
 |
| 出版日期 | 2006 |
| 卷号 | 176期号:40547页码:185-199 |
| 关键词 | degradation 1-methylindole 3-methylindole mangrove sediment Pseudomonas aeruginosa Gs first-order kinetic model |
| ISSN号 | 0049-6979 |
| 通讯作者 | jdgu@hkucc.hku.hk |
| 中文摘要 | Indolic compounds are N-heterocyclic aromatic chemicals and have been detected at contaminated sites. Biodegradation of 1-methylindole (1MI) and 3-methylindole (3MI) was investigated initially using enrichment cultures with mangrove sediment obtained from Mai Po Nature Reserve of Hong Kong and subsequently with a pure culture of Pseudomonas aeruginosa Gs confirmed with 16S rRNA gene. At 2.0 mM, 1MI and 3MI were degraded in 4 and 3 days, respectively, by the respective 1MI- and 3MI-degrading enrichment cultures. When substrate concentrations were increased to 3.0,mM and 3.5,mM, slower degradation of 1MI and 3MI was observed indicating inhibitory effects from the substrates, possibly due to toxicity. In addition, no colony of bacteria could be observed on the agar plates amended with 3.5,mM 1MI or 4.0,mM 3MI, indicating that 1MI was more toxic than 3MI. Pseudomonas aeruginosa Gs, isolated from the enrichment culture, effectively utilized both substrates as the sole source of carbon and energy. Complete degradation of 1MI and 3MI was achieved after more than 40 days and 24 days, respectively, at an initial concentration of 2,mM in the culture. Effects of initial substrate concentration, pH and salinity on degradation of 1MI and 3MI by P. aeruginosa Gs were also studied in batch culture. The optimum pH and salinity for degrading both substrates by P. aeruginosa Gs was 7.0 and 5 parts per thousand, respectively. Biodegradation kinetics of 1MI and 3MI by P. aeruginosa Gs could be described using a first-order kinetic model. Our results suggest that both 1MI and 3MI are biodegradable in the mangrove environment and that toxicity of 1MI could be a potential factor limiting the removal of the chemical in the environment by microorganisms. |
| 学科主题 | Environmental Sciences; Meteorology & Atmospheric Sciences; Water Resources |
| 收录类别 | SCI |
| 原文出处 | SPRINGER |
| WOS记录号 | WOS:000240318000012 |
| 公开日期 | 2011-07-03 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/5773]  |
| 专题 | 南海海洋研究所_热带海洋环境国家重点实验室(LTO) |
| 推荐引用方式 GB/T 7714 | Yin, B,Huang, LM,Gu, JD. Biodegradation of 1-methylindole and 3-methylindole by mangrove sediment enrichment cultures and a pure culture of an isolated Pseudomonas aeruginosa Gs[J]. WATER AIR AND SOIL POLLUTION,2006,176(40547):185-199. |
| APA | Yin, B,Huang, LM,&Gu, JD.(2006).Biodegradation of 1-methylindole and 3-methylindole by mangrove sediment enrichment cultures and a pure culture of an isolated Pseudomonas aeruginosa Gs.WATER AIR AND SOIL POLLUTION,176(40547),185-199. |
| MLA | Yin, B,et al."Biodegradation of 1-methylindole and 3-methylindole by mangrove sediment enrichment cultures and a pure culture of an isolated Pseudomonas aeruginosa Gs".WATER AIR AND SOIL POLLUTION 176.40547(2006):185-199. |
入库方式： OAI收割
来源：南海海洋研究所
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