Proteomic analysis during larval development and metamorphosis of the spionid polychaete Pseudopolydora vexillosa
文献类型：期刊论文
| 作者 | Mok, FSY ; Thiyagarajan, V ; Qian, PY |
| 刊名 | PROTEOME SCIENCE
 |
| 出版日期 | 2009 |
| 卷号 | 7页码:- |
| ISSN号 | 1477-5956 |
| 通讯作者 | floramok@ust.hk ; rajan@hkucc.hku.hk ; boqianpy@ust.hk |
| 中文摘要 | Background: While the larval-juvenile transition (metamorphosis) in the spionid polychaete Pseudopolydora vexillosa involves gradual morphological changes and does not require substantial development of juvenile organs, the opposite occurs in the barnacle Balanus amphitrite. We hypothesized that the proteome changes during metamorphosis in the spionids are less drastic than that in the barnacles. To test this, proteomes of pre-competent larvae, competent larvae (ready to metamorphose), and juveniles of P. vexillosa were compared using 2-dimensional gel electrophoresis (2-DE), and they were then compared to those of the barnacle. Results: Unlike the significant changes found during barnacle metamorphosis, proteomes of competent P. vexillosa larvae were more similar to those of their juveniles. Pre-competent larvae had significantly fewer protein spots (384 spots), while both competent larvae and juveniles expressed about 660 protein spots each. Proteins up-regulated during competence identified by MALDI-TOF/TOF analysis included a molecular chaperon (calreticulin), a signal transduction regulator (tyrosin activation protein), and a tissue-remodeling enzyme (metallopeptidase). Conclusions: This was the first time to study the protein expression patterns during the metamorphosis of a marine polychaete and to compare the proteomes of marine invertebrates that have different levels of morphological changes during metamorphosis. The findings provide promising initial steps towards the development of a proteome database for marine invertebrate metamorphosis, thus deciphering the possible mechanisms underlying larval metamorphosis in non-model marine organisms. |
| 学科主题 | Biochemical Research Methods |
| 收录类别 | SCI |
| 原文出处 | BIOMED CENTRAL LTD |
| WOS记录号 | WOS:000273444500001 |
| 公开日期 | 2011-07-03 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/6049]  |
| 专题 | 南海海洋研究所_热带海洋环境国家重点实验室(LTO) |
| 推荐引用方式 GB/T 7714 | Mok, FSY,Thiyagarajan, V,Qian, PY. Proteomic analysis during larval development and metamorphosis of the spionid polychaete Pseudopolydora vexillosa[J]. PROTEOME SCIENCE,2009,7:-. |
| APA | Mok, FSY,Thiyagarajan, V,&Qian, PY.(2009).Proteomic analysis during larval development and metamorphosis of the spionid polychaete Pseudopolydora vexillosa.PROTEOME SCIENCE,7,-. |
| MLA | Mok, FSY,et al."Proteomic analysis during larval development and metamorphosis of the spionid polychaete Pseudopolydora vexillosa".PROTEOME SCIENCE 7(2009):-. |
入库方式： OAI收割
来源：南海海洋研究所
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