Expression analysis of type 2 metallothionein gene in mangrove species (Bruguiera gymnorrhiza) under heavy metal stress
文献类型：期刊论文
| 作者 | Huang, GY ; Wang, YS |
| 刊名 | CHEMOSPHERE
 |
| 出版日期 | 2009 |
| 卷号 | 77期号:7页码:1026-1029 |
| 关键词 | Copper Lead Metallothioneins Reverse transcription Zinc |
| ISSN号 | 0045-6535 |
| 通讯作者 | huang_gyh@sina.com |
| 中文摘要 | In this paper, we aimed to assess the roles of metallothioneins (MTs) in heavy metal tolerance by analyzing the expression level of BgM72 in leaves of Bruguiera gymnorrhiza in response to heavy metals. Eight-month-old B. gymnorrhiza seedlings were exposed to different concentrations of zinc (Zn), copper (Cu) or lead (Pb) for 1, 3 and 7 d. A Real-time quantitative PCR protocol was developed to directly evaluate the expression of BgMT2, using 18S rRNA as a reference gene. Real-time quantitative PCR analysis demonstrated BgMT2 mRNA expression was regulated by Zn, Cu and Pb, but the regulation pattern was different for the three metals tested. Significant increase in the transcript level of BgM72 was also found in response to Zn, Cu and Pb in some experimental conditions. Our results confirm that BgMT2 gene is involved in the regulation of Zn, Cu and Pb in B. gymnorrhiza leaves. (C) 2009 Elsevier Ltd. All rights reserved. |
| 学科主题 | Environmental Sciences |
| 收录类别 | SCI |
| 原文出处 | PERGAMON-ELSEVIER SCIENCE LTD |
| WOS记录号 | WOS:000271992200021 |
| 公开日期 | 2011-07-03 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/6065]  |
| 专题 | 南海海洋研究所_热带海洋环境国家重点实验室(LTO) |
| 推荐引用方式 GB/T 7714 | Huang, GY,Wang, YS. Expression analysis of type 2 metallothionein gene in mangrove species (Bruguiera gymnorrhiza) under heavy metal stress[J]. CHEMOSPHERE,2009,77(7):1026-1029. |
| APA | Huang, GY,&Wang, YS.(2009).Expression analysis of type 2 metallothionein gene in mangrove species (Bruguiera gymnorrhiza) under heavy metal stress.CHEMOSPHERE,77(7),1026-1029. |
| MLA | Huang, GY,et al."Expression analysis of type 2 metallothionein gene in mangrove species (Bruguiera gymnorrhiza) under heavy metal stress".CHEMOSPHERE 77.7(2009):1026-1029. |
入库方式： OAI收割
来源：南海海洋研究所
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