Identification of a C-type lectin from the bay scallop Argopecten irradians
文献类型：期刊论文
| 作者 | Zhu, L ; Song, LS ; Xu, W ; Qian, PY |
| 刊名 | MOLECULAR BIOLOGY REPORTS
 |
| 出版日期 | 2009 |
| 卷号 | 36期号:5页码:1167-1173 |
| 关键词 | C-type lectin CRD Argopecten irradians Tissue distribution mRNA expression Immune response |
| ISSN号 | 0301-4851 |
| 通讯作者 | llingzhu@163.com ; boqianpy@ust.hk |
| 中文摘要 | C-type lectins are Ca2+ dependent carbohydrate-recognition proteins that play crucial roles in the invertebrate innate immunity, such as nonself recognition, activation of proPO system, antibacterial activity, promotion of phagocytosis and nodule formation. In this study, a novel C-type lectin of bay scallops Argopecten irradians (Ai Lec) was identified using expressed sequence tag (EST) and RACE techniques. The Ai Lec cDNA encoded a polypeptide of 171 amino acids with a putative signal peptide of 21 amino acid residues and a mature protein of 150 amino acids. The deduced amino acid sequence of Ai Lec was highly similar to those of the C-type lectins from other animals and contained a typical carbohydrate-recognition domain (CRD) of 131 residues, which has four conserved disulfide-bonded cysteine residues that define the CRD and two additional cysteine residues at the amino terminus. The expression of Ai Lec transcript was dominantly detected in the hepatopancreas and slightly detected in the haemocytes of normal scallops. 6 h after Vibrio anguillarum-challenge and 8 h after Micrococcus luteus-challenge, the temporal expression of Ai Lec mRNA in hemocytes was increased by 4.4- and 3.6-folds, respectively. The results suggested that Ai Lec was a constitutive and inducible acute-phase protein and might be involved in immune response to Gram-negative and Gram-positive microbial infection in bay scallop A. irradians. |
| 学科主题 | Biochemistry & Molecular Biology |
| 收录类别 | SCI |
| 原文出处 | SPRINGER |
| WOS记录号 | WOS:000264851600042 |
| 公开日期 | 2011-07-03 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/6129]  |
| 专题 | 南海海洋研究所_热带海洋环境国家重点实验室(LTO) |
| 推荐引用方式 GB/T 7714 | Zhu, L,Song, LS,Xu, W,et al. Identification of a C-type lectin from the bay scallop Argopecten irradians[J]. MOLECULAR BIOLOGY REPORTS,2009,36(5):1167-1173. |
| APA | Zhu, L,Song, LS,Xu, W,&Qian, PY.(2009).Identification of a C-type lectin from the bay scallop Argopecten irradians.MOLECULAR BIOLOGY REPORTS,36(5),1167-1173. |
| MLA | Zhu, L,et al."Identification of a C-type lectin from the bay scallop Argopecten irradians".MOLECULAR BIOLOGY REPORTS 36.5(2009):1167-1173. |
入库方式： OAI收割
来源：南海海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。