Expression and characterization analysis of type 2 metallothionein from grey mangrove species (Avicennia marina) in response to metal stress
文献类型：期刊论文
| 作者 | Huang, GY ; Wang, YS |
| 刊名 | AQUATIC TOXICOLOGY
 |
| 出版日期 | 2010 |
| 卷号 | 99期号:1页码:86-92 |
| 关键词 | Metals GST fusion protein Metallothionein Reverse transcription |
| ISSN号 | 0166-445X |
| 通讯作者 | huang_gyh@sina.com |
| 中文摘要 | Metallothioneins (MTs) are a family of low-molecular-weight cysteine-rich proteins and are thought to play possible roles in metal metabolism or detoxification. To evaluate the roles of metallothioneins in metal homeostasis or tolerance in Avicennia marina, a real-time quantitative PCR protocol was developed to directly evaluate the expression of AmMT2 mRNA, when A. marina seedlings were exposed to different concentrations of zinc (Zn), copper (Cu) or lead (Pb) for 3 and 7d. Real-time quantitative PCR results indicated that the regulation of AmMT2 mRNA expression by Zn, Cu and Pb was strongly dependent on concentration and time of exposure. A significant increase in the transcripts of AmMT2 gene was also found in response to Zn, Cu and Pb, at least under some experimental conditions. When AmMT2 was overexpressed in Escherichia coli BL21 as a carboxy-terminal extension of glutathione-S-transferase (GST), the transgenic bacteria showed an increased tolerance to Zn, Cu, Pb and Cd exposure as compared to control strains. Moreover, GST-AmMT2 was purified from E. coli cells grown in the presence of 400 mu M Zn, Cu, Pb or Cd. The purified GST-AmMT2 fusion protein could bind higher levels of all four metals than GST alone. Taken together, these data support the hypothesis that AmMT2 may be involved in processes of metal homeostasis or tolerance in A. marina. (C) 2010 Elsevier B.V. All rights reserved. |
| 学科主题 | Marine & Freshwater Biology; Toxicology |
| 收录类别 | SCI |
| 原文出处 | ELSEVIER SCIENCE BV |
| WOS记录号 | WOS:000279277800009 |
| 公开日期 | 2011-07-03 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/6283]  |
| 专题 | 南海海洋研究所_热带海洋环境国家重点实验室(LTO) |
| 推荐引用方式 GB/T 7714 | Huang, GY,Wang, YS. Expression and characterization analysis of type 2 metallothionein from grey mangrove species (Avicennia marina) in response to metal stress[J]. AQUATIC TOXICOLOGY,2010,99(1):86-92. |
| APA | Huang, GY,&Wang, YS.(2010).Expression and characterization analysis of type 2 metallothionein from grey mangrove species (Avicennia marina) in response to metal stress.AQUATIC TOXICOLOGY,99(1),86-92. |
| MLA | Huang, GY,et al."Expression and characterization analysis of type 2 metallothionein from grey mangrove species (Avicennia marina) in response to metal stress".AQUATIC TOXICOLOGY 99.1(2010):86-92. |
入库方式： OAI收割
来源：南海海洋研究所
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