Loop-mediated isothermal amplification integrated on microfluidic chips for point-of-care quantitative detection of pathogens
文献类型：期刊论文
| 作者 | Fang, Xueen1,2,3; Liu, Yingyi3; Kong, Jilie1,2; Jiang, Xingyu3 |
| 刊名 | Analytical chemistry
 |
| 出版日期 | 2010-04-01 |
| 卷号 | 82期号:7页码:3002-3006 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/ac1000652 |
| 通讯作者 | Kong, jilie(jlkong@fudan.edu.cn) |
| 英文摘要 | This work shows that loop-mediated isothermal amplification (lamp) of nucleic acid can be integrated in an eight-channel microfluidic chip for readout either by the naked eye (as a result of the insoluble byproduct pyrophosphate generating during lamp amplification) or via absorbance measured by an optic sensor; we call this system micro-lamp (mu lamp). it is capable of analyzing target nucleic acids quantitatively with high sensitivity and specificity. the assay is straightforward in manipulation. it requires a sample volume of 0.4 mu l and is complete within 1 h. the sensitivity of the assay is comparable to standard methods, where 10 fg of dna sample could be detected under isothermal conditions (63 degrees c). a real time quantitative mu lamp assay using absorbance detection is possible by integration of optical fibers within the chip. |
| WOS关键词 | PSEUDORABIES VIRUS ; AUJESZKYS-DISEASE ; VISUAL DETECTION ; LAMP ; DNA ; PIGS ; ELECTROPHORESIS ; IMMUNOASSAYS ; MEMBRANES ; SYSTEM |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000276004000055 |
| 出版者 | AMER CHEMICAL SOC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2176013 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Kong, Jilie |
| 作者单位 | 1.Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China 2.Fudan Univ, Inst Biomed Sci, Shanghai 200433, Peoples R China 3.Natl Ctr Nanosci & Technol, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fang, Xueen,Liu, Yingyi,Kong, Jilie,et al. Loop-mediated isothermal amplification integrated on microfluidic chips for point-of-care quantitative detection of pathogens[J]. Analytical chemistry,2010,82(7):3002-3006. |
| APA | Fang, Xueen,Liu, Yingyi,Kong, Jilie,&Jiang, Xingyu.(2010).Loop-mediated isothermal amplification integrated on microfluidic chips for point-of-care quantitative detection of pathogens.Analytical chemistry,82(7),3002-3006. |
| MLA | Fang, Xueen,et al."Loop-mediated isothermal amplification integrated on microfluidic chips for point-of-care quantitative detection of pathogens".Analytical chemistry 82.7(2010):3002-3006. |
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来源：高能物理研究所
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