Binding modes of thioflavin t molecules to prion peptide assemblies identified by using scanning tunneling microscopy
文献类型：期刊论文
| 作者 | Mao, Xiaobo1,2; Guo, Yuanyuan1,2; Wang, Chenxuan1,2; Zhang, Min1,2; Ma, Xiaojing1,2; Liu, Lei1,2; Niu, Lin1,2; Zeng, Qingdao1,2; Yang, Yanlian1,2; Wang, Chen1,2 |
| 刊名 | Acs chemical neuroscience
 |
| 出版日期 | 2011-06-01 |
| 卷号 | 2期号:6页码:281-287 |
| 关键词 | Gnnqqny Thioflavin t Binding mode Amyloid Labeling molecule Scanning tunneling microscopy |
| ISSN号 | 1948-7193 |
| DOI | 10.1021/cn200006h |
| 通讯作者 | Yang, yanlian(yangyl@nanoctr.cn) |
| 英文摘要 | The widely used method to monitor the aggregation process of amyloid peptide is thioflavin t (tht) assay, while the detailed molecular mechanism is still not clear. in this work, we report here the direct identification of the binding modes of tht molecules with the prion peptide gnnqqny by using scanning tunneling microscopy (stm). the assembly structures of gnnqqny were first observed by stm on a graphite surface, and the introduction of tht molecules to the surface facilitated the stm observations of the adsorption conformations of tht with peptide strands. tht molecules are apt to adsorb on the peptide assembly with beta-sheet structure and oriented parallel with the peptide strands adopting four different binding modes. this effort could benefit the understanding of the mechanisms of the interactions between labeling species or inhibitory ligands and amyloid peptides, which is keenly needed for developing diagnostic and therapeutic approaches. |
| WOS关键词 | AMYLOID FIBRILS ; ALZHEIMERS-DISEASE ; CONGO RED ; DYNAMICS SIMULATIONS ; HIGH-AFFINITY ; PROTEIN ; MECHANISM ; DERIVATIVES ; INHIBITION ; TOXICITY |
| WOS研究方向 | Biochemistry & Molecular Biology ; Pharmacology & Pharmacy ; Neurosciences & Neurology |
| WOS类目 | Biochemistry & Molecular Biology ; Chemistry, Medicinal ; Neurosciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000291896800003 |
| 出版者 | AMER CHEMICAL SOC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2176071 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Yang, Yanlian |
| 作者单位 | 1.Chinese Acad Sci, Natl Ctr Nanoscience & Technol, Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Natl Ctr Nanoscience & Technol, Key Lab Standardizat & Measurement Nanotechnol, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Mao, Xiaobo,Guo, Yuanyuan,Wang, Chenxuan,et al. Binding modes of thioflavin t molecules to prion peptide assemblies identified by using scanning tunneling microscopy[J]. Acs chemical neuroscience,2011,2(6):281-287. |
| APA | Mao, Xiaobo.,Guo, Yuanyuan.,Wang, Chenxuan.,Zhang, Min.,Ma, Xiaojing.,...&Wang, Chen.(2011).Binding modes of thioflavin t molecules to prion peptide assemblies identified by using scanning tunneling microscopy.Acs chemical neuroscience,2(6),281-287. |
| MLA | Mao, Xiaobo,et al."Binding modes of thioflavin t molecules to prion peptide assemblies identified by using scanning tunneling microscopy".Acs chemical neuroscience 2.6(2011):281-287. |
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来源：高能物理研究所
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