Binding of blood proteins to carbon nanotubes reduces cytotoxicity
文献类型：期刊论文
| 作者 | Ge, Cuicui2; Du, Jiangfeng2; Zhao, Lina2; Wang, Liming2; Liu, Ying2; Li, Denghua2; Yang, Yanlian2; Zhou, Ruhong1,3; Zhao, Yuliang2,4; Chai, Zhifang4 |
| 刊名 | Proceedings of the national academy of sciences of the united states of america
 |
| 出版日期 | 2011-10-11 |
| 卷号 | 108期号:41页码:16968-16973 |
| 关键词 | Competitive adsorption Nanoparticle-protein corona Hydrophobic interactions Conformational flexibility |
| ISSN号 | 0027-8424 |
| DOI | 10.1073/pnas.1105270108 |
| 通讯作者 | Zhou, ruhong(ruhongz@us.ibm.com) |
| 英文摘要 | With the potential wide uses of nanoparticles such as carbon nanotubes in biomedical applications, and the growing concerns of nanotoxicity of these engineered nanoparticles, the importance of nanoparticle-protein interactions cannot be stressed enough. in this study, we use both experimental and theoretical approaches, including atomic force microscope images, fluorescence spectroscopy, cd, sds-page, and molecular dynamics simulations, to investigate the interactions of single-wall carbon nanotubes (swcnts) with human serum proteins, and find a competitive binding of these proteins with different adsorption capacity and packing modes. the p-p stacking interactions between swcnts and aromatic residues (trp, phe, tyr) are found to play a critical role in determining their adsorption capacity. additional cellular cytotoxicity assays, with human acute monocytic leukemia cell line and human umbilical vein endothelial cells, reveal that the competitive bindings of blood proteins on the swcnt surface can greatly alter their cellular interaction pathways and result in much reduced cytotoxicity for these protein-coated swcnts, according to their respective adsorption capacity. these findings have shed light toward the design of safe carbon nanotube nanomaterials by comprehensive preconsideration of their interactions with human serum proteins. |
| WOS关键词 | ATOMIC-FORCE MICROSCOPY ; AQUEOUS-SOLUTION ; WATER ; NANOPARTICLES ; CELLS ; MICE ; NANOMEDICINE ; SPECTROSCOPY ; COLLAPSE ; DELIVERY |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000295973800025 |
| 出版者 | NATL ACAD SCIENCES |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2176090 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Zhou, Ruhong |
| 作者单位 | 1.IBM Corp, Thomas J Watson Res Ctr, Computat Biol Ctr, Yorktown Hts, NY 10598 USA 2.Chinese Acad Sci, Inst High Energy Phys, Key Lab Biomed Effects Nanomat & Nanosafety, Natl Ctr Nanosci & Technol, Beijing 100190, Peoples R China 3.Columbia Univ, Dept Chem, New York, NY 10027 USA 4.Chinese Acad Sci, Inst High Energy Phys, Key Lab Nucl Analyt Tech, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ge, Cuicui,Du, Jiangfeng,Zhao, Lina,et al. Binding of blood proteins to carbon nanotubes reduces cytotoxicity[J]. Proceedings of the national academy of sciences of the united states of america,2011,108(41):16968-16973. |
| APA | Ge, Cuicui.,Du, Jiangfeng.,Zhao, Lina.,Wang, Liming.,Liu, Ying.,...&Chen, Chunying.(2011).Binding of blood proteins to carbon nanotubes reduces cytotoxicity.Proceedings of the national academy of sciences of the united states of america,108(41),16968-16973. |
| MLA | Ge, Cuicui,et al."Binding of blood proteins to carbon nanotubes reduces cytotoxicity".Proceedings of the national academy of sciences of the united states of america 108.41(2011):16968-16973. |
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