Gold nanoparticles induce autophagosome accumulation through size-dependent nanoparticle uptake and lysosome impairment
文献类型：期刊论文
| 作者 | Ma, Xiaowei2,3; Wu, Yanyang1; Jin, Shubin2,3; Tian, Yuan5; Zhang, Xiaoning4; Zhao, Yuliang2,3; Yu, Li1; Liang, Xing-Jie2,3 |
| 刊名 | Acs nano
 |
| 出版日期 | 2011-11-01 |
| 卷号 | 5期号:11页码:8629-8639 |
| 关键词 | Gold nanoparticles (aunps) Autophagosome accumulation Autophagic flux Lysosome impairment Lysosomal ph |
| ISSN号 | 1936-0851 |
| DOI | 10.1021/nn202155y |
| 通讯作者 | Yu, li(liyulab@mail.tsinghua.edu.cn) |
| 英文摘要 | Development of nanotechnology calls for a comprehensive understanding of the impact of nanomaterials on biological systems. autophagy is a lysosome-based degradative pathway which plays an essential role in maintaining cellular homeostasis. previous studies have shown that nanoparticles from various sources can induce autophagosome accumulation in treated cells. however, the underlying mechanism is still not clear. gold nanoparticles (aunps) are one of the most widely used nanomaterials and have been reported to induce autophagosome accumulation. in this study, we found that aunps can be taken into cells through endocytosis in a size-dependent manner. the internalized aunps eventually accumulate in lysosomes and cause impairment of lysosome degradation capacity through alkalinization of lysosomal ph. consistent with previous studies, we found that aunp treatment can induce autophagosome accumulation and processing of lc3, an autophagosome marker protein. however, degradation of the autophagy substrate p62 is blocked in aunp-treated cells, which indicates that autophagosome accumulation results from blockade of autophagy flux, rather than induction of autophagy. our data clarify the mechanism by which aunps induce autophagosome accumulation and reveal the effect of aunps on lysosomes. this work is significant to nanoparticle research because it illustrates how nanoparticles can potentially interrupt the autophagic pathway and has important implications for biomedical applications of nanoparticles. |
| WOS关键词 | CELL-DEATH ; ENDOPLASMIC-RETICULUM ; QUANTUM DOTS ; ENDOCYTOSIS ; PH ; PACLITAXEL ; SURVIVAL ; MACROPHAGES ; METABOLISM ; ACTIVATION |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Materials Science |
| WOS类目 | Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary |
| 语种 | 英语 |
| WOS记录号 | WOS:000297143300020 |
| 出版者 | AMER CHEMICAL SOC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2176133 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Yu, Li |
| 作者单位 | 1.Tsinghua Univ, Sch Life Sci, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100084, Peoples R China 2.Natl Ctr Nanosci & Technol, Div Nanomed & Nanobiol, Lab Nanomed & Nanosafety, Beijing, Peoples R China 3.Chinese Acad Sci, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China 4.Tsinghua Univ, Coll Med, Lab Pharmaceut, Beijing 100084, Peoples R China 5.China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100193, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Xiaowei,Wu, Yanyang,Jin, Shubin,et al. Gold nanoparticles induce autophagosome accumulation through size-dependent nanoparticle uptake and lysosome impairment[J]. Acs nano,2011,5(11):8629-8639. |
| APA | Ma, Xiaowei.,Wu, Yanyang.,Jin, Shubin.,Tian, Yuan.,Zhang, Xiaoning.,...&Liang, Xing-Jie.(2011).Gold nanoparticles induce autophagosome accumulation through size-dependent nanoparticle uptake and lysosome impairment.Acs nano,5(11),8629-8639. |
| MLA | Ma, Xiaowei,et al."Gold nanoparticles induce autophagosome accumulation through size-dependent nanoparticle uptake and lysosome impairment".Acs nano 5.11(2011):8629-8639. |
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来源：高能物理研究所
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