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Variation in the internalization of differently sized nanoparticles induces different dna-damaging effects on a macrophage cell line

文献类型:期刊论文

作者Zhang, Mingyi1; Li, Juan1; Xing, Gengmei1; He, Rui2; Li, Wei1; Song, Yan1; Guo, Haili1
刊名Archives of toxicology
出版日期2011-12-01
卷号85期号:12页码:1575-1588
关键词Dna damage Entry pathway Carboxylated nanoparticles Size-dependency Raw 264.7
ISSN号0340-5761
DOI10.1007/s00204-011-0725-y
通讯作者Xing, gengmei(xinggm@ihep.ac.cn)
英文摘要Although researchers have expended considerable effort on studying the cytotoxicity of nanomaterials, it is possible that there has been insufficient attention paid to their genotoxic potential. here, we describe a test model that we have developed to evaluate the dna-damaging effects of negatively charged nanoparticles of different sizes. we compared the dna damaging effect induced by nanoparticles of various sizes and found that the effect is closely associated with the internalization pattern of the particles. macrophage cell line raw 264.7 cells were incubated with carboxylated polystyrene beads (cooh-pbs) ranging in size from 30 to 500 nm. size-dependent dna damage was detected, and the lesion induced by two carboxylated fullerene particles confirmed this observation. confocal microscopy revealed that the entry pathways of these cooh-pbs shifted from direct penetration to endocytosis with increasing particle size, followed by changes in subcellular localization. subsequent deposition of 30-nm cooh-pbs in the cytosol led to a reduction of zn(2+) and mg(2+) content in the nucleus and an increased p53 level in the whole cell rather than in nucleus, while localization of 50- and 100-nm cooh-pbs in acidic vesicles induced p53 accumulation in both types of extracts. based on these results, we assume that the damage resulted from a disruption of the balance between dna damage and repair.
WOS关键词GEL-ELECTROPHORESIS ; SURFACE-CHEMISTRY ; P53 ; ENDOCYTOSIS ; REPAIR ; ZINC ; PHAGOCYTOSIS ; DISPERSIONS ; FULLERENES ; PARTICLES
WOS研究方向Toxicology
WOS类目Toxicology
语种英语
WOS记录号WOS:000297359700012
出版者SPRINGER HEIDELBERG
URI标识http://www.irgrid.ac.cn/handle/1471x/2176159
专题高能物理研究所
通讯作者Xing, Gengmei
作者单位1.Chinese Acad Sci, Lab Bioenvironm Effects Nanomat & Nanosafety, Inst High Energy Phys, Beijing 10049, Peoples R China
2.Hangzhou Normal Univ, Res Ctr Biomed & Hlth, Hangzhou 310012, Zhejiang, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Mingyi,Li, Juan,Xing, Gengmei,et al. Variation in the internalization of differently sized nanoparticles induces different dna-damaging effects on a macrophage cell line[J]. Archives of toxicology,2011,85(12):1575-1588.
APA Zhang, Mingyi.,Li, Juan.,Xing, Gengmei.,He, Rui.,Li, Wei.,...&Guo, Haili.(2011).Variation in the internalization of differently sized nanoparticles induces different dna-damaging effects on a macrophage cell line.Archives of toxicology,85(12),1575-1588.
MLA Zhang, Mingyi,et al."Variation in the internalization of differently sized nanoparticles induces different dna-damaging effects on a macrophage cell line".Archives of toxicology 85.12(2011):1575-1588.

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