Predicting viruses accurately by a multiplex microfluidic loop-mediated isothermal amplification chip
文献类型：期刊论文
| 作者 | Fang, Xueen1,2,3; Chen, Hui2,3; Yu, Shaoning2,3; Jiang, Xingyu1; Kong, Jilie2,3 |
| 刊名 | Analytical chemistry
 |
| 出版日期 | 2011-02-01 |
| 卷号 | 83期号:3页码:690-695 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/ac102858j |
| 通讯作者 | Jiang, xingyu(xingyujiang@nanoctr.cn) |
| 英文摘要 | Multiplex gene assay is a valuable molecular tool not only in academic science but also in clinical diagnostics. multiplex pcr assays, dna microarrays, and various nanotechnology-based methods are examples of major techniques developed for analyzing multiple genes; none of these, however, are suitable for point-of-care diagnostics, especially in resource limited settings. in this report, we describe an octopus like multiplex microfluidic loop mediated isothermal amplification (m mu lamp) assay for the rapid analysis of multiple genes in the point-of-care format and provide a robust approach for predicting viruses. this assay with the ability: of analyzing multiple genes qualitatively and quantitatively is highly specific, operationally simple, and cost/time-effective with the detection limit of less than 10 copies/mu l in 2 mu l quantities of sample within 0.5 h. we successfully developed a m mu lamp chip for differentiating three human influenza a substrains and identifying eight important swine viruses. |
| WOS关键词 | PCR ; DNA ; TECHNOLOGY ; IDENTIFICATION |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000286689600012 |
| 出版者 | AMER CHEMICAL SOC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2176175 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Jiang, Xingyu |
| 作者单位 | 1.Natl Ctr Nanosci & Technol, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China 2.Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China 3.Fudan Univ, Inst Biomed Sci, Shanghai 200433, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fang, Xueen,Chen, Hui,Yu, Shaoning,et al. Predicting viruses accurately by a multiplex microfluidic loop-mediated isothermal amplification chip[J]. Analytical chemistry,2011,83(3):690-695. |
| APA | Fang, Xueen,Chen, Hui,Yu, Shaoning,Jiang, Xingyu,&Kong, Jilie.(2011).Predicting viruses accurately by a multiplex microfluidic loop-mediated isothermal amplification chip.Analytical chemistry,83(3),690-695. |
| MLA | Fang, Xueen,et al."Predicting viruses accurately by a multiplex microfluidic loop-mediated isothermal amplification chip".Analytical chemistry 83.3(2011):690-695. |
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来源：高能物理研究所
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