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Isolation and characterization of a novel sulfur-oxidizing chemolithoautotroph halothiobacillus from pb polluted paddy soil

文献类型:期刊论文

作者Shi, Jiyan1; Lin, Huirong1,2; Yuan, Xiaofeng3; Zhao, Yidong4
刊名African journal of biotechnology
出版日期2011-05-16
卷号10期号:20页码:4121-4126
关键词Halothiobacillus Heavy-metals-tolerant Soxb gene 16s rrna gene Sulfur globule
ISSN号1684-5315
通讯作者Lin, huirong(linhuirong@yahoo.com.cn)
英文摘要A new mesophilic and chemolithoautotrophic sulfur oxidizing bacterium (sob) strain ht1 was isolated from a rice rhizosphere soil polluted by pb using thiosulfate as electron donor at ph 7.0. the 16s rrna gene sequence showed that the new isolate was a sulfur oxidizing obligate chemolithotroph belonging to gammaproteobacteria, halothiobacillus and utilizing different reduced sulfur compounds (sulfide, elemental sulfur, thiosulfate and sulfite) as chemolithotrophic substrates. strain ht1 was able to use co(2) as a carbon source responsible for the reduction of nitrate to nitrite, which represented a halophilic sob capable of growth within a broad salinity range of 0 to 3 m nacl and a heavy-metals-tolerant sob. ht1 was gram negative, motile and was proposed as the type strain of a novel species of sulfur oxidizing bacteria. the soxb gene could not be detected in strain ht1 during thiosulfate oxidation. the metabolism pathway of ht1 was 's4 intermediate' (s4i) pathway. sulfur globules accumulated in ht1 were mainly s(8).
WOS关键词NEAR-EDGE SPECTROSCOPY ; MOLECULAR CHARACTERIZATION ; SP NOV. ; THIOSULFATE ; BACTERIA ; OXIDATION ; GENE ; THIOBACILLUS ; METABOLISM ; SOXB
WOS研究方向Biotechnology & Applied Microbiology
WOS类目Biotechnology & Applied Microbiology
语种英语
WOS记录号WOS:000294612200024
出版者ACADEMIC JOURNALS
URI标识http://www.irgrid.ac.cn/handle/1471x/2176235
专题高能物理研究所
通讯作者Lin, Huirong
作者单位1.Zhejiang Univ, Inst Environm Sci & Technol, Hangzhou 310029, Zhejiang, Peoples R China
2.Xiamen Univ, Tan Kah Kee Coll, Dept Environm Sci & Engn, Zhangzhou 363105, Peoples R China
3.Zhejiang Chinese Med Univ, Dept Life Sci, Hangzhou 310053, Zhejiang, Peoples R China
4.Chinese Acad Sci, Inst High Energy Phys, Beijing Synchrotron Radiat Facil, Beijing 100049, Peoples R China
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Shi, Jiyan,Lin, Huirong,Yuan, Xiaofeng,et al. Isolation and characterization of a novel sulfur-oxidizing chemolithoautotroph halothiobacillus from pb polluted paddy soil[J]. African journal of biotechnology,2011,10(20):4121-4126.
APA Shi, Jiyan,Lin, Huirong,Yuan, Xiaofeng,&Zhao, Yidong.(2011).Isolation and characterization of a novel sulfur-oxidizing chemolithoautotroph halothiobacillus from pb polluted paddy soil.African journal of biotechnology,10(20),4121-4126.
MLA Shi, Jiyan,et al."Isolation and characterization of a novel sulfur-oxidizing chemolithoautotroph halothiobacillus from pb polluted paddy soil".African journal of biotechnology 10.20(2011):4121-4126.

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