Nano-cage-mediated refolding of insulin by peg-pe micelle
文献类型：期刊论文
| 作者 | Fang, Xiaocui1,2; Yang, Tao1,3; Wang, Luoyang1,3; Yu, Jibing1; Wei, Xiuli1; Zhou, Yinjian1; Wang, Chen2; Liang, Wei1 |
| 刊名 | Biomaterials
 |
| 出版日期 | 2016 |
| 卷号 | 77页码:139-148 |
| 关键词 | Insulin Dtt Refolding Peg-pe micelle Nano-cage Negative charge layer |
| ISSN号 | 0142-9612 |
| DOI | 10.1016/j.biomaterials.2015.11.007 |
| 通讯作者 | Wang, chen(wangch@nanoctr.cn) |
| 英文摘要 | Insulin aggregation has pronounced pharmaceutical implications and biological importance. deposition of insulin aggregates is associated with type ii diabetes and instability of pharmaceutical formulations. we present in this study the renaturation effect of peg-pe micelle on dithiothreitol (dtt)-denatured insulin revealed by techniques including turbidity assay, circular dichroism (cd), thioflavint (tht) binding assay, bis-ans binding assay, agarose gel electrophoresis and maldi-tof ms. the obtained results show that peg-pe micelle having a hydrophilic nano-cage-like structure in which with a negative charge layer, can capture dtt-induced insulin a and b chains, and block their hydrophobic interaction, thereby preventing aggregation. the reduced insulin a and b chain in the nano-cage are capable of recognizing each other and form the native insulin with yields of similar to 30% as measured by hypoglycemic activity analysis in mice. the observed insulin refolding assisted by peg-pe micelle may be applicable to other proteins. (c) 2015 published by elsevier ltd. |
| WOS关键词 | PROTEIN DISULFIDE-ISOMERASE ; CARBONIC ANHYDRASE-B ; AMYLOID FIBRILS ; IN-VIVO ; PHOSPHOLIPID MICELLES ; MOLECULAR CHAPERONES ; POLYMERIC MICELLES ; LIQUID INTERFACES ; DRUG-DELIVERY ; AGGREGATION |
| WOS研究方向 | Engineering ; Materials Science |
| WOS类目 | Engineering, Biomedical ; Materials Science, Biomaterials |
| 语种 | 英语 |
| WOS记录号 | WOS:000367118200013 |
| 出版者 | ELSEVIER SCI LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2176433 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Wang, Chen |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, Prot & Peptide Pharmaceut Lab, Beijing 100101, Peoples R China 2.Natl Ctr Nanosci & Technol, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fang, Xiaocui,Yang, Tao,Wang, Luoyang,et al. Nano-cage-mediated refolding of insulin by peg-pe micelle[J]. Biomaterials,2016,77:139-148. |
| APA | Fang, Xiaocui.,Yang, Tao.,Wang, Luoyang.,Yu, Jibing.,Wei, Xiuli.,...&Liang, Wei.(2016).Nano-cage-mediated refolding of insulin by peg-pe micelle.Biomaterials,77,139-148. |
| MLA | Fang, Xiaocui,et al."Nano-cage-mediated refolding of insulin by peg-pe micelle".Biomaterials 77(2016):139-148. |
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来源：高能物理研究所
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