Label-free isolation and mrna detection of circulating tumor cells from patients with metastatic lung cancer for disease diagnosis and monitoring therapeutic efficacy
文献类型：期刊论文
| 作者 | Wang, Jidong1,2; Lu, Wenjing1,2; Tang, Chuanhao3; Liu, Yi3; Sun, Jiashu1,2; Mu, Xuan4; Zhang, Lin4; Dai, Bo5; Li, Xiaoyan3; Zhuo, Hailong3 |
| 刊名 | Analytical chemistry
 |
| 出版日期 | 2015-12-01 |
| 卷号 | 87期号:23页码:11893-11900 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.5b03484 |
| 通讯作者 | Sun, jiashu(sunjs@nanoctr.cn) |
| 英文摘要 | We develop an inertial-based microfluidic cell sorter combined with an integrated membrane filter, allowing for size-based, label-free, and high-efficiency separation and enrichment of circulating tumor cells (ctcs) in whole blood. the cell sorter is composed of a double spiral microchannel that hydrodynamically focuses and separates large ctcs from small blood cells. the focused ctcs with the equilibrium position around the midline of microchannel are further captured and enriched by a membrane filter (pore size of 8 mu m) attached at the middle outlet. this integrated microfluidic device can process 1 ml of whole blood containing spiked tumor cells (a549, human lung adenocarcinoma epithelial cell line) within 15 min, with the capture efficiency of 74.4% at the concentration as low as tens of a549 cells per ml of whole blood. this microfluidic cell sorter is further adopted for isolation of ctcs from peripheral blood samples of patients with metastatic lung cancer. the immunostaining and ck-19 mrna detection are applied for identification of captured ctcs, showing that our method can detect 90% of metastatic lung cancer patients before therapy, whereas the commercially used system can only detect 40% of the same patients. we also use the expression of ck-19 mrna from captured ctcs as an indicator for monitoring the therapeutic efficiency, which correlates well with x-ray computed tomography (ct) assessment of the disease. |
| WOS关键词 | POLYMERASE-CHAIN-REACTION ; MICROFLUIDIC DEVICE ; PERIPHERAL-BLOOD ; SEPARATION ; MICROCHANNELS ; NANOPARTICLES ; AMPLIFICATION ; TECHNOLOGY ; PARTICLES ; CAPTURE |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000365931100041 |
| 出版者 | AMER CHEMICAL SOC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2176434 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Sun, Jiashu |
| 作者单位 | 1.Natl Ctr Nanosci & Technol, Beijing Engn Res Ctr BioNanotechnol, Beijing 100190, Peoples R China 2.Natl Ctr Nanosci & Technol, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China 3.Affiliated Hosp Acad Mil Med Sci, Hosp 307, Beijing 100071, Peoples R China 4.Chinese Acad Med Sci, Peking Union Med Coll, Beijing 100730, Peoples R China 5.Fudan Univ, Shanghai Canc Ctr, Dept Urol, Shanghai 200032, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Jidong,Lu, Wenjing,Tang, Chuanhao,et al. Label-free isolation and mrna detection of circulating tumor cells from patients with metastatic lung cancer for disease diagnosis and monitoring therapeutic efficacy[J]. Analytical chemistry,2015,87(23):11893-11900. |
| APA | Wang, Jidong.,Lu, Wenjing.,Tang, Chuanhao.,Liu, Yi.,Sun, Jiashu.,...&Jiang, Xingyu.(2015).Label-free isolation and mrna detection of circulating tumor cells from patients with metastatic lung cancer for disease diagnosis and monitoring therapeutic efficacy.Analytical chemistry,87(23),11893-11900. |
| MLA | Wang, Jidong,et al."Label-free isolation and mrna detection of circulating tumor cells from patients with metastatic lung cancer for disease diagnosis and monitoring therapeutic efficacy".Analytical chemistry 87.23(2015):11893-11900. |
入库方式： iSwitch采集
来源：高能物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。