Response of human osteoblast to n-ha/peek-quantitative proteomic study of bio-effects of nano-hydroxyapatite composite
文献类型：期刊论文
| 作者 | Zhao, Minzhi1; Li, Haiyun1; Liu, Xiaochen4; Wei, Jie5; Ji, Jianguo3; Yang, Shu1; Hu, Zhiyuan1; Wei, Shicheng2,4 |
| 刊名 | Scientific reports
 |
| 出版日期 | 2016-03-09 |
| 卷号 | 6页码:13 |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/srep22832 |
| 通讯作者 | Hu, zhiyuan(huzy@nanoctr.cn) ; Wei, shicheng(sc-wei@pku.edu.cn) |
| 英文摘要 | Nano-sized hydroxyapatite (n-ha) is considered as a bio-active material, which is often mixed into bone implant material, polyetheretherketone (peek). to reveal the global protein expression modulations of osteoblast in response to direct contact with the peek composite containing high level (40%) nanosized hydroxyapatite (n-ha/peek) and explain its comprehensive bio-effects, quantitative proteomic analysis was conducted on human osteoblast-like cells mg-63 cultured on n-ha/peek in comparison with pure peek. results from quantitative proteomic analysis showed that the most enriched categories in the up-regulated proteins were related to calcium ion processes and associated functions while the most enriched categories in the down-regulated proteins were related to rna process. this enhanced our understanding to the molecular mechanism of the promotion of the cell adhesion and differentiation with the inhibition of the cell proliferation on n-ha/peek composite. it also exhibited that although the calcium ion level of incubate environment hadn't increased, merely the calcium fixed on the surface of material had influence to intracellular calcium related processes, which was also reflect by the higher intracellular ca2+ concentration of n-ha/peek. this study could lead to more comprehensive cognition to the versatile biocompatibility of composite materials. it further proves that proteomics is useful in new bio-effect discovery. |
| WOS关键词 | ACTIN-BINDING PROTEIN ; GLYCOPROTEIN IB-ALPHA ; IN-VITRO ; DARIER-DISEASE ; POLYETHERETHERKETONE ; ADHESION ; FILAMIN ; PEEK ; GENE ; DIFFERENTIATION |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000371605200002 |
| 出版者 | NATURE PUBLISHING GROUP |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2176545 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Hu, Zhiyuan; Wei, Shicheng |
| 作者单位 | 1.Chinese Acad Sci, Natl Ctr Nanosci & Technol, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China 2.Peking Univ, Sch & Hosp Stomatol, Ctr Craniofacial Stem Cell Res & Regenerat, Dept Orthodont,Lab Interdisciplinary Studies, Beijing 100081, Peoples R China 3.Peking Univ, Coll Life Sci, State Key Lab Prot & Plant Gene Res, Beijing 100871, Peoples R China 4.Peking Univ, Acad Adv Interdisciplinary Studies, Ctr Biomed Mat & Tissue Engn, Beijing 100871, Peoples R China 5.E China Univ Sci & Technol, Minist Educ, Key Lab Ultrafine Mat, Shanghai 200237, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Minzhi,Li, Haiyun,Liu, Xiaochen,et al. Response of human osteoblast to n-ha/peek-quantitative proteomic study of bio-effects of nano-hydroxyapatite composite[J]. Scientific reports,2016,6:13. |
| APA | Zhao, Minzhi.,Li, Haiyun.,Liu, Xiaochen.,Wei, Jie.,Ji, Jianguo.,...&Wei, Shicheng.(2016).Response of human osteoblast to n-ha/peek-quantitative proteomic study of bio-effects of nano-hydroxyapatite composite.Scientific reports,6,13. |
| MLA | Zhao, Minzhi,et al."Response of human osteoblast to n-ha/peek-quantitative proteomic study of bio-effects of nano-hydroxyapatite composite".Scientific reports 6(2016):13. |
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来源：高能物理研究所
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