Tumor acidity-induced sheddable polyethylenimine-poly(trimethylene carbonate)/dna/polyethylene glycol-2,3-dimethylmaleicanhydride ternary complex for efficient and safe gene delivery
文献类型：期刊论文
| 作者 | Zhao, Caiyan; Shao, Leihou; Lu, Jianqing; Deng, Xiongwei; Wu, Yan |
| 刊名 | Acs applied materials & interfaces
 |
| 出版日期 | 2016-03-16 |
| 卷号 | 8期号:10页码:6400-6410 |
| 关键词 | Sheddable Tumor acidity Pegylation Polyethylenimine-poly(trimethylene carbonate) Gene delivery |
| ISSN号 | 1944-8244 |
| DOI | 10.1021/acsami.6b00825 |
| 通讯作者 | Wu, yan(wuy@nanoctr.cn) |
| 英文摘要 | Amphiphilic pei derivatives/dna complexes are widely used for dna delivery, but they are unstable in vivo and have cytotoxicity due to the excess cationic charge. pegylation of cationic complexes can improve sterical stability and biocompatibility. however, pegylation significantly inhibits cellular uptake and endosomal escape. in this work, sheddable ternary complexes were developed by coating a tumor acidity-sensitive beta-carboxylic amide functionalized peg layer on the binary complexes of amphiphilic cationic polyethylenimine-poly(trimethylene carbonate) nanoparticles/dna (pei-ptmc/dna). such sheddable ternary complexes markedly reduced their nonspecific interactions with serum protein in the bloodstream and obtained minimal cytotoxicity due to the protection of the peg shell. at the tumor site, the peg layer was deshielded by responding to the tumor acidic microenvironment and the positively charged complexes re-exposed that had higher affinity with negatively charged cell membranes. meanwhile the positively charged complexes facilitated endosomal escape. accordingly, this delivery system improved the biocompatibility of gene-loaded complexes and enhanced the gene transfection efficiency. such pegylated complexes with the ability to deshield the peg layer at the target tissues hold great promise for efficient and safe gene delivery in vivo. |
| WOS关键词 | TARGETED SIRNA DELIVERY ; POLY(TRIMETHYLENE CARBONATE) ; IN-VITRO ; CANCER-THERAPY ; DRUG-DELIVERY ; NANOPARTICLES ; PH ; POLYETHYLENIMINE ; TRANSFECTION ; COPOLYMERS |
| WOS研究方向 | Science & Technology - Other Topics ; Materials Science |
| WOS类目 | Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary |
| 语种 | 英语 |
| WOS记录号 | WOS:000372479300013 |
| 出版者 | AMER CHEMICAL SOC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2176555 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Wu, Yan |
| 作者单位 | Natl Ctr Nanosci & Technol, CAS Ctr Excellence Nanosci, CAS Key Lab Biomed Effects Nanomat & Nanosafety, 11 Beiyitiao, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Caiyan,Shao, Leihou,Lu, Jianqing,et al. Tumor acidity-induced sheddable polyethylenimine-poly(trimethylene carbonate)/dna/polyethylene glycol-2,3-dimethylmaleicanhydride ternary complex for efficient and safe gene delivery[J]. Acs applied materials & interfaces,2016,8(10):6400-6410. |
| APA | Zhao, Caiyan,Shao, Leihou,Lu, Jianqing,Deng, Xiongwei,&Wu, Yan.(2016).Tumor acidity-induced sheddable polyethylenimine-poly(trimethylene carbonate)/dna/polyethylene glycol-2,3-dimethylmaleicanhydride ternary complex for efficient and safe gene delivery.Acs applied materials & interfaces,8(10),6400-6410. |
| MLA | Zhao, Caiyan,et al."Tumor acidity-induced sheddable polyethylenimine-poly(trimethylene carbonate)/dna/polyethylene glycol-2,3-dimethylmaleicanhydride ternary complex for efficient and safe gene delivery".Acs applied materials & interfaces 8.10(2016):6400-6410. |
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来源：高能物理研究所
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