Selective detection of 8-oxo-2'-deoxyguanosine in single-stranded dna via nanopore sensing approach
文献类型：期刊论文
| 作者 | Liu, Lei1; Li, Yuru1; Li, Ting1; Xie, Jiani1; Chen, Chaofei2; Liu, Quansheng2; Zhang, Shouwen3; Wu, Hai -Chen1 |
| 刊名 | Analytical chemistry
 |
| 出版日期 | 2016-01-19 |
| 卷号 | 88期号:2页码:1073-1077 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.5b04102 |
| 通讯作者 | Liu, lei(leiliu@ihep.ac.cn) ; Wu, hai -chen(haichenwu@ihep.ac.cn) |
| 英文摘要 | We have developed a nanopore sensing approach for the selective detection of 8-oxo-2'-deoxyguanosine (8-oxog) in single-stranded dna. first, 1,12-dodecanediamine is coupled with 8-oxog-containing dna molecules in high yield which leaves a free amine group for subsequent attaching of an adamantane moiety. after incubation with cucurbit[7]uril, the host-guest complex-modified dna hybrid is translocated through an alpha-hemolysin nanopore. highly characteristic events can be recorded and used to quantify the 8-oxog-dna content in a dna mixture. compared with the existing methods, this study provides a reliable, quick, and low-cost approach for the detection of 8-oxog site in single-stranded dna at the single-molecule level, particularly suitable for high-throughput screening of a massive number of samples. |
| WOS关键词 | ALPHA-HEMOLYSIN NANOPORE ; HOST-GUEST INTERACTIONS ; MOLECULE DETECTION ; OXIDATIVE DAMAGE ; COMET ASSAY ; PROTEIN ; 5-HYDROXYMETHYLCYTOSINE ; 8-OXOGUANINE ; PORE ; 8-OXO-2'-DEOXYGUANOSINE |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000368562300002 |
| 出版者 | AMER CHEMICAL SOC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2176570 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Liu, Lei; Wu, Hai -Chen |
| 作者单位 | 1.Chinese Acad Sci, Inst High Energy Phys, Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst High Energy Phys, Multidisciplinary Ctr, Beijing 100049, Peoples R China 3.Beijing ChaoYang Emergency Med Ctr, Epilepsy Dept, Beijing 100021, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Lei,Li, Yuru,Li, Ting,et al. Selective detection of 8-oxo-2'-deoxyguanosine in single-stranded dna via nanopore sensing approach[J]. Analytical chemistry,2016,88(2):1073-1077. |
| APA | Liu, Lei.,Li, Yuru.,Li, Ting.,Xie, Jiani.,Chen, Chaofei.,...&Wu, Hai -Chen.(2016).Selective detection of 8-oxo-2'-deoxyguanosine in single-stranded dna via nanopore sensing approach.Analytical chemistry,88(2),1073-1077. |
| MLA | Liu, Lei,et al."Selective detection of 8-oxo-2'-deoxyguanosine in single-stranded dna via nanopore sensing approach".Analytical chemistry 88.2(2016):1073-1077. |
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来源：高能物理研究所
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