Quantitative detection of microrna in one step via next generation magnetic relaxation switch sensing
文献类型：期刊论文
| 作者 | Lu, Wenjing1,2; Chen, Yiping1,2; Liu, Zhong3; Tang, Wenbo4; Feng, Qiang1,2; Sun, Jiashu1,2; Jiang, Xingyu1,2 |
| 刊名 | Acs nano
 |
| 出版日期 | 2016-07-01 |
| 卷号 | 10期号:7页码:6685-6692 |
| 关键词 | Mirna Magnetic relaxation switch Magnetic nanoparticles Detection Tumor |
| ISSN号 | 1936-0851 |
| DOI | 10.1021/acsnano.6b01903 |
| 通讯作者 | Sun, jiashu(sunjs@nanoctr.cn) ; Jiang, xingyu(xingyujiang@nanoctr.cn) |
| 英文摘要 | One-step, quantitative and rapid detection of microrna (mirna) in tumor cells or tissues can provide critical information for clinical diagnosis and cancer treatment. in this work, we develop a magnetic relaxation switch sensing (mrs)-based mirna sensor using magnetic microparticle (1 mu m in diameter, mm1000)-dna probe-magnetic nanoparticle (30 nm in diameter, mn3(0)) conjugates (mm1000-dna-mn30). in the presence of target mirna, dsn enzyme selectively cleaves the dna tether after mirna/dna hybridization to release mn30 and leaves the mirna intact to lead to the declustering of more mn30 than before. in contrast to conventional mrs by measuring the change of transverse relaxation time (delta t-2) induced by the aggregation or dissociation of magnetic particles in the presence of target, we use the cleaved mn30 from conjugates as the direct readout of delta t-2, which is more sensitive and stable. this mrs-based assay allows for one-step detection of 5 fm of mir-21 in urine samples, quantification of mir-21 from 100 cancer cells, and differentiation of the expression of mir-21 in tumor and surrounding tissues. the merits of this assay, rapidity, ability for quantitation, high sensitivity, and one-step operation, ensure a promising future in diagnostic technology. |
| WOS关键词 | ISOTHERMAL AMPLIFICATION ; CANCER-CELLS ; SENSITIVE DETECTION ; DIAGNOSTIC ASSAY ; NUCLEIC-ACIDS ; LUNG-CANCER ; NANOPARTICLES ; PROBES ; MICROCAPILLARY ; SEQUENCES |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Materials Science |
| WOS类目 | Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary |
| 语种 | 英语 |
| WOS记录号 | WOS:000380576600031 |
| 出版者 | AMER CHEMICAL SOC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2176592 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Sun, Jiashu; Jiang, Xingyu |
| 作者单位 | 1.Natl Ctr NanoSci & Technol, Beijing Engn Res Ctr BioNanotechnol, CAS Ctr Excellence Nanosci, Beijing 100190, Peoples R China 2.Natl Ctr NanoSci & Technol, CAS Key Lab Biol Effects Nanomat & Nanosafety, CAS Ctr Excellence Nanosci, Beijing 100190, Peoples R China 3.Chinese Acad Med Sci, Peking Union Med Coll, Inst Blood Transfus, Beijing 100730, Peoples R China 4.Chinese Peoples Liberat Army Gen Hosp, Dept Hepatopancreaticobiliary Surg Oncol, Beijing 100853, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu, Wenjing,Chen, Yiping,Liu, Zhong,et al. Quantitative detection of microrna in one step via next generation magnetic relaxation switch sensing[J]. Acs nano,2016,10(7):6685-6692. |
| APA | Lu, Wenjing.,Chen, Yiping.,Liu, Zhong.,Tang, Wenbo.,Feng, Qiang.,...&Jiang, Xingyu.(2016).Quantitative detection of microrna in one step via next generation magnetic relaxation switch sensing.Acs nano,10(7),6685-6692. |
| MLA | Lu, Wenjing,et al."Quantitative detection of microrna in one step via next generation magnetic relaxation switch sensing".Acs nano 10.7(2016):6685-6692. |
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来源：高能物理研究所
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