Peptidic beta-sheet binding with congo red allows both reduction of error variance and signal amplification for immunoassays
文献类型：期刊论文
| 作者 | Wang, Yunyun1,2,3,4; Liu, Ye2,3,4; Deng, Xinli1; Cong, Yulong1; Jiang, Xingyu2,3,4 |
| 刊名 | Biosensors & bioelectronics
 |
| 出版日期 | 2016-12-15 |
| 卷号 | 86页码:211-218 |
| 关键词 | Peptidic beta-sheet Congo red P-selectin Readout mode Immunoassays |
| ISSN号 | 0956-5663 |
| DOI | 10.1016/j.bios.2016.06.021 |
| 通讯作者 | Cong, yulong(yulongc@263.net) ; Jiang, xingyu(xingyujiang@nanoctr.cn) |
| 英文摘要 | Although conventional enzyme-linked immunosorbent assays (elisa) and related assays have been widely applied for the diagnosis of diseases, many of them suffer from large error variance for monitoring the concentration of targets over time, and insufficient limit of detection (lod) for assaying dilute targets. we herein report a readout mode of elisa based on the binding between peptidic beta-sheet structure and congo red: the formation of peptidic beta-sheet structure is triggered by alkaline phosphatase (alp). for the detection of p-selectin which is a crucial indicator for evaluating thrombus diseases in clinic, the 'beta-sheet and congo red' mode significantly decreases both the error variance and, the lod (from 9.7 ng/ml to 1.1 ng/ml) of detection, compared with commercial elisa (an existing gold-standard method for detecting p-selectin in clinic). considering the wide range of alp-based antibodies for immunoassays, such novel method could be applicable to the analysis of many types of targets. (c) 2016 elsevier b.v. all rights reserved. |
| WOS关键词 | SOLUBLE P-SELECTIN ; ADHESION ; FLUORESCENCE ; ANTAGONIST ; MOLECULES ; DIAGNOSIS ; THERAPY ; DISEASE ; VACCINE ; PROTEIN |
| WOS研究方向 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
| WOS类目 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology |
| 语种 | 英语 |
| WOS记录号 | WOS:000384853300031 |
| 出版者 | ELSEVIER ADVANCED TECHNOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2176637 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Cong, Yulong; Jiang, Xingyu |
| 作者单位 | 1.Chinese Peoples Liberat Army Gen Hosp, Clin Lab South Bldg, 28 Fuxing Rd, Beijing 100853, Peoples R China 2.Natl Ctr NanoSci & Technol, Beijing Engn Res Ctr BioNanotechnol, 11 Zhongguancun Beijing, Beijing 100190, Peoples R China 3.Natl Ctr NanoSci & Technol, CAS Key Lab Biol Effects Nanomat & Nanosafety, CAS Ctr Excellence Nanosci, 11 Zhongguancun Beijing, Beijing 100190, Peoples R China 4.Univ Chinese Acad Sci, 11 Zhongguancun Beijing, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yunyun,Liu, Ye,Deng, Xinli,et al. Peptidic beta-sheet binding with congo red allows both reduction of error variance and signal amplification for immunoassays[J]. Biosensors & bioelectronics,2016,86:211-218. |
| APA | Wang, Yunyun,Liu, Ye,Deng, Xinli,Cong, Yulong,&Jiang, Xingyu.(2016).Peptidic beta-sheet binding with congo red allows both reduction of error variance and signal amplification for immunoassays.Biosensors & bioelectronics,86,211-218. |
| MLA | Wang, Yunyun,et al."Peptidic beta-sheet binding with congo red allows both reduction of error variance and signal amplification for immunoassays".Biosensors & bioelectronics 86(2016):211-218. |
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来源：高能物理研究所
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