Microfluidic chip-based immunoassay for reliable detection of cloxacillin in poultry
文献类型：期刊论文
| 作者 | Yu, Wenbo1; Chen, Yiping2,3; Knauer, Maria1; Dietrich, Richard1; Maertlbauer, Erwin1; Jiang, Xingyu2,3 |
| 刊名 | Food analytical methods
 |
| 出版日期 | 2016-11-01 |
| 卷号 | 9期号:11页码:3163-3169 |
| 关键词 | Microfluidic chips Chemiluminescence Immunoassay Cloxacillin Poultry |
| ISSN号 | 1936-9751 |
| DOI | 10.1007/s12161-016-0508-4 |
| 通讯作者 | Maertlbauer, erwin(e.maertlbauer@lmu.de) ; Jiang, xingyu(xingyujiang@nanoctr.cn) |
| 英文摘要 | By combining a chemiluminescence immunoassay with microfluidic chip technology, a simple assay was established for the sensitive detection of cloxacillin in poultry samples. the chip used in this approach was composed of an upper microchannel layer and a lower flat base layer. each of the formed microchannels has a volume of 18 mu l, thus enabling a considerable reduction of reagent consumption as well as sample size needed for analysis by the indirect competitive immunoassay. to obtain labeled antigens for coating, the active ester method was employed to couple cloxacillin to glucose oxidase (glcox). the immobilized glcox-cloxacillin conjugate on the base layer competed with residues of cloxacillin in samples for binding sites of the monoclonal antibodies (mab) against cloxacillin. horseradish peroxidase-labeled anti-mouse igg antibodies and chemiluminescent substrate were used for signal generation. under optimized conditions, the ic50 and the limit of detection (lod) of the assay were at 96.5 +/- 9.37 and 0.92 +/- 0.07 ng/ml, respectively, and thus well below the maximum residue level of 300 ng/ml set by the european commission. recoveries for spiked chicken and duck samples were in the range from 94 to 118 % with relative standard deviations lower than 11 %. the results demonstrate that the microfluidic chip-based immunoassay can be used as a rapid and reliable platform for determination of cloxacillin in poultry. |
| WOS关键词 | BETA-LACTAM ANTIBIOTICS ; LIQUID-CHROMATOGRAPHY ; ALPHA-FETOPROTEIN ; MILK ; FOOD ; AMPLIFICATION ; PENICILLINS ; RESIDUES ; MUSCLE ; DEVICE |
| WOS研究方向 | Food Science & Technology |
| WOS类目 | Food Science & Technology |
| 语种 | 英语 |
| WOS记录号 | WOS:000385192700021 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2176642 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Maertlbauer, Erwin; Jiang, Xingyu |
| 作者单位 | 1.Univ Munich, Dept Vet Sci, Chair Hyg & Technol Milk, Schonleutnerstr 8, D-85764 Oberschleissheim, Germany 2.Natl Ctr NanoSci & Technol, Beijing Engn Res Ctr BioNanotechnol, 11 ZhongGuanCun BeiYiTiao, Beijing 100190, Peoples R China 3.Natl Ctr NanoSci & Technol, Key Lab Biol Effects Nanomat & Nanosafety, 11 ZhongGuanCun BeiYiTiao, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Wenbo,Chen, Yiping,Knauer, Maria,et al. Microfluidic chip-based immunoassay for reliable detection of cloxacillin in poultry[J]. Food analytical methods,2016,9(11):3163-3169. |
| APA | Yu, Wenbo,Chen, Yiping,Knauer, Maria,Dietrich, Richard,Maertlbauer, Erwin,&Jiang, Xingyu.(2016).Microfluidic chip-based immunoassay for reliable detection of cloxacillin in poultry.Food analytical methods,9(11),3163-3169. |
| MLA | Yu, Wenbo,et al."Microfluidic chip-based immunoassay for reliable detection of cloxacillin in poultry".Food analytical methods 9.11(2016):3163-3169. |
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来源：高能物理研究所
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