One-step multiplexed detection of foodborne pathogens: combining a quantum dot-mediated reverse assaying strategy and magnetic separation
文献类型：期刊论文
| 作者 | Yin, Binfeng1,2,6; Wang, Yu4,5; Dong, Mingling1,2; Wu, Jing3; Ran, Bei1,2; Xie, Mengxia3; Joo, Sang Woo6; Chen, Yiping1,2 |
| 刊名 | Biosensors & bioelectronics
 |
| 出版日期 | 2016-12-15 |
| 卷号 | 86页码:996-1002 |
| 关键词 | Reverse assaying strategy Quantum dot One-step detection Multiplexed detection |
| ISSN号 | 0956-5663 |
| DOI | 10.1016/j.bios.2016.07.106 |
| 通讯作者 | Xie, mengxia(xiemx@bnu.edu.cn) ; Joo, sang woo(swjoo@yu.ac.kr) ; Chen, yiping(chenyp@nanoctr.cn) |
| 英文摘要 | A rapid and multiplexed immunosensor was developed based on a quantum dot (qd)-reverse assaying strategy (ras) and immuno-magnetic beads (imbs) for one-step and simultaneous detection of escherichia coli o157: h7 and salmonella. in a conventional qd-based immunosensor, the fluorescence signal of the "imbs-target-qd" immunoconjugate is directly used as the assaying readout. however, the fluorescence signal is affected by imbs due to light scattering and the "imbs-target-qd" immunoconjugate needs multiple washing and re-suspension steps. to address these problems, we use the surplus qd-antibody conjugate as signal readout in the ras, which prevents interference from the imbs, increases the fluorescence signal, and avoids complex operations. compared with conventional qd-based immunosensor, the sensitivity of qd-rsa immunosensor for detection of escherichia coli o157: h7 has been improved fifty-fold, and whole analysis procedure can be finished within 1 h. therefore, this rsa strategy is promising for improving the performance of qd-based immunosensors and could greatly broaden their applications. (c) 2016 elsevier b.v. all rights reserved. |
| WOS关键词 | SALMONELLA-TYPHIMURIUM ; UNITED-STATES ; IMMUNOMAGNETIC SEPARATION ; COXSACKIEVIRUS B3 ; RAPID DETECTION ; ENTEROVIRUS 71 ; FOOD ; CELLS ; IMMUNOASSAY |
| WOS研究方向 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
| WOS类目 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology |
| 语种 | 英语 |
| WOS记录号 | WOS:000384853300138 |
| 出版者 | ELSEVIER ADVANCED TECHNOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2176643 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Xie, Mengxia; Joo, Sang Woo; Chen, Yiping |
| 作者单位 | 1.Natl Ctr NanoSci & Technol, CAS Ctr Excellence Nanosci, Beijing Engn Res Ctr BioNanotechnol, Beijing 100190, Peoples R China 2.Natl Ctr NanoSci & Technol, CAS Ctr Excellence Nanosci, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China 3.Beijing Normal Univ, Analyt & Testing Ctr, Beijing 100875, Peoples R China 4.Capital Med Univ, Beijing Friendship Hosp, 95 Yongan Rd, Beijing 100050, Peoples R China 5.Beijing Inst Trop Med, Beijing 100050, Peoples R China 6.Yeungnam Univ, Sch Mech Engn, Kyongsan 712749, South Korea |
| 推荐引用方式 GB/T 7714 | Yin, Binfeng,Wang, Yu,Dong, Mingling,et al. One-step multiplexed detection of foodborne pathogens: combining a quantum dot-mediated reverse assaying strategy and magnetic separation[J]. Biosensors & bioelectronics,2016,86:996-1002. |
| APA | Yin, Binfeng.,Wang, Yu.,Dong, Mingling.,Wu, Jing.,Ran, Bei.,...&Chen, Yiping.(2016).One-step multiplexed detection of foodborne pathogens: combining a quantum dot-mediated reverse assaying strategy and magnetic separation.Biosensors & bioelectronics,86,996-1002. |
| MLA | Yin, Binfeng,et al."One-step multiplexed detection of foodborne pathogens: combining a quantum dot-mediated reverse assaying strategy and magnetic separation".Biosensors & bioelectronics 86(2016):996-1002. |
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来源：高能物理研究所
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