Enhanced fluorescence elisa based on hat triggering fluorescence "turn-on" with enzyme-antibody dual labeled aunp probes for ultrasensitive detection of afp and hbsag
文献类型：期刊论文
| 作者 | Wu, Yudong1,2; Guo, Weisheng3; Peng, Weipan1,2; Zhao, Qan1,2; Piao, Jiafang1,2; Zhang, Bo1,2; Wu, Xiaoli1,2; Wang, Hanjie1,2; Gong, Xiaoqun1,2; Chang, Jin1,2 |
| 刊名 | Acs applied materials & interfaces
 |
| 出版日期 | 2017-03-22 |
| 卷号 | 9期号:11页码:9369-9377 |
| 关键词 | Fluorescence enzyme-linked immunosorbent assay Gold nanoparticles Human alpha-thrombin Alpha fetoprotein Hepatitis b virus surface antigen |
| ISSN号 | 1944-8244 |
| DOI | 10.1021/acsami.6b16236 |
| 通讯作者 | Gong, xiaoqun(gongxiaoqun@tju.edu.cn) ; Chang, jin(jinchang@tju.edu.cn) |
| 英文摘要 | At present, enzyme-linked immunosorbent assay (elisa) is considered to be the most appropriate approach in clinical biomarker detection, with good specificity, low cost, arid straightforward readout. however, unsatisfactory sensitivity severely hampers its wide application in clinical diagnosis. herein, we designed a new kind of enhanced fluorescence enzyme-inked immunosorbent assay (felisa) based on the human alpha-thrombin (hat) triggering fluorescence "turn-on" signals. in this system, detection antibodies (ab(2)) and hat were labeled on the gold nanoparticles (aunps) to form the detection probes, and a bisamide derivative of rhodamineno(110) with fluorescence quenched served as the substrate of hat. after the sandwich immunoreaction, hat on the sandwich structure could catalyze the cleavage of the fluorescence-quenched substrate, leading to a strong fluorescence signal for sensing ultralow levels of alpha fetoprotein (afp) and hepatitis b virus surface antigen (hbsag). under the optimized reaction conditions, afp and hbsag were detected at the ultralow concentrations of 10(-8) ng ml(-1) and 5 x 10(-4) iu ml(-i), respectively, which were at least 10(4) times lower than those of the conventional fluorescence assay and 106 times lower than those of the conventional elisa. in addition, we further discussed the efficiency of the sensitive felisa in clinical serum samples, showing great potential in practical applications. |
| WOS关键词 | LINKED-IMMUNOSORBENT-ASSAY ; PROSTATE-SPECIFIC ANTIGEN ; GOLD NANOPARTICLES ; SENSITIVE DETECTION ; ALPHA-FETOPROTEIN ; CHEMILUMINESCENCE IMMUNOASSAY ; IMMUNOCHROMATOGRAPHIC ASSAY ; SIGNAL AMPLIFICATION ; SILVER NANOPARTICLES ; PLASMONIC ELISA |
| WOS研究方向 | Science & Technology - Other Topics ; Materials Science |
| WOS类目 | Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary |
| 语种 | 英语 |
| WOS记录号 | WOS:000397478100020 |
| 出版者 | AMER CHEMICAL SOC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2176683 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Gong, Xiaoqun; Chang, Jin |
| 作者单位 | 1.Tianjin Univ, Sch Life Sci, Sch Mat Sci & Engn, 92 Weijin Rd, Tianjin 300072, Peoples R China 2.Tianjin Engn Ctr Micro Nano Biomat & Detect Treat, 92 Weijin Rd, Tianjin 300072, Peoples R China 3.Natl Ctr Nanosci & Technol, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Yudong,Guo, Weisheng,Peng, Weipan,et al. Enhanced fluorescence elisa based on hat triggering fluorescence "turn-on" with enzyme-antibody dual labeled aunp probes for ultrasensitive detection of afp and hbsag[J]. Acs applied materials & interfaces,2017,9(11):9369-9377. |
| APA | Wu, Yudong.,Guo, Weisheng.,Peng, Weipan.,Zhao, Qan.,Piao, Jiafang.,...&Chang, Jin.(2017).Enhanced fluorescence elisa based on hat triggering fluorescence "turn-on" with enzyme-antibody dual labeled aunp probes for ultrasensitive detection of afp and hbsag.Acs applied materials & interfaces,9(11),9369-9377. |
| MLA | Wu, Yudong,et al."Enhanced fluorescence elisa based on hat triggering fluorescence "turn-on" with enzyme-antibody dual labeled aunp probes for ultrasensitive detection of afp and hbsag".Acs applied materials & interfaces 9.11(2017):9369-9377. |
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来源：高能物理研究所
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