Peptidic beta-sheets induce congo red-derived fluorescence to improve the sensitivity of hiv-1 p24 detection
文献类型：期刊论文
| 作者 | Sun, Hongyan1,2; Liu, Ye2; Zhang, Cuntong1; Luo, Xuegang1; Jiang, Xingyu2,3 |
| 刊名 | Analytical methods
 |
| 出版日期 | 2017-02-21 |
| 卷号 | 9期号:7页码:1185-1189 |
| ISSN号 | 1759-9660 |
| DOI | 10.1039/c6ay03489f |
| 通讯作者 | Luo, xuegang(luoxuegang@tust.edu.cn) ; Jiang, xingyu(xingyujiang@nanoctr.cn) |
| 英文摘要 | At the early stage of hiv infection, the low concentration of hiv components seriously hinders the diagnosis of this fatal disease. improving the sensitivity of detection thus becomes the only approach to effectively detect hiv infection at the early stages. we use alkaline phosphatase to catalyze short peptides that contain a 2-phenylalanine core component (2f) to form beta-sheets which can combine with congo red to produce a strong fluorescent signal. for the detection of hiv-1 p24, the limit of detection of 2f-congo red reaches 2.44 pg ml(-1), which is lower than that of clinical elisa (5 pg ml(-1)). the inter-batch variability, intra-batch variability and precision of 2f-congo red for hiv detection are 5.4%, 2.3% and 2.9%, respectively, which are similar to those of clinical elisa ( 4.1%, 2.7% and 3.2%, respectively). the variability and precision of 2f- congo red is consistent to that of clinical elisa for quantifying hiv-1 p24. 2f- congo red thus shows a better performance for the detection of hiv infection at the early stages, as detection by clinical elisa is difficult at the early stages of infection. |
| WOS关键词 | DIAGNOSIS ; VACCINE |
| WOS研究方向 | Chemistry ; Food Science & Technology ; Spectroscopy |
| WOS类目 | Chemistry, Analytical ; Food Science & Technology ; Spectroscopy |
| 语种 | 英语 |
| WOS记录号 | WOS:000395684800014 |
| 出版者 | ROYAL SOC CHEMISTRY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2176713 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Luo, Xuegang; Jiang, Xingyu |
| 作者单位 | 1.Tianjin Univ Sci & Technol, Key Lab Ind Fermentat Microbiol, Tianjin Key Lab Ind Microbiol, Minist Educ,Coll Biotechnol, Tianjin 300457, Peoples R China 2.Natl Ctr NanoSci & Technol, CAS Ctr Excellence Nanosci, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing Engn Res Ctr BioNanotechnol, 11 Zhongguancun Beiyitiao, Beijing 100190, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Hongyan,Liu, Ye,Zhang, Cuntong,et al. Peptidic beta-sheets induce congo red-derived fluorescence to improve the sensitivity of hiv-1 p24 detection[J]. Analytical methods,2017,9(7):1185-1189. |
| APA | Sun, Hongyan,Liu, Ye,Zhang, Cuntong,Luo, Xuegang,&Jiang, Xingyu.(2017).Peptidic beta-sheets induce congo red-derived fluorescence to improve the sensitivity of hiv-1 p24 detection.Analytical methods,9(7),1185-1189. |
| MLA | Sun, Hongyan,et al."Peptidic beta-sheets induce congo red-derived fluorescence to improve the sensitivity of hiv-1 p24 detection".Analytical methods 9.7(2017):1185-1189. |
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来源：高能物理研究所
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