A tyrosinase-triggered oxidative reaction-based "turn-on" fluorescent probe for imaging in living melanoma cells
文献类型：期刊论文
| 作者 | Li, Zhipeng1; Wang, Yi-Feng2,3,4; Zhang, Xu5,6,7; Zeng, Chengchu1; Hu, Liming1,8; Liang, Xing-Jie2,3 |
| 刊名 | Sensors and actuators b-chemical
 |
| 出版日期 | 2017-04-01 |
| 卷号 | 242页码:189-194 |
| 关键词 | Tyrosinase Fluorescent probe High sensitivity Turn-on |
| ISSN号 | 0925-4005 |
| DOI | 10.1016/j.snb.2016.11.011 |
| 通讯作者 | Hu, liming(huliming@bjut.edu.cn) ; Liang, xing-jie(liangxj@nanoctr.cn) |
| 英文摘要 | Melanoma, with poor prognosis and highly metastatic spread, is the most deadly skin cancer, but the monitoring and diagnosis of melanoma is still a challenging. the rate-limiting enzyme tyrosinase is crucial for controlling melanin production, and is a well-known biomarker closely associated with the level of malignancy. however, effective probes for detecting tyrosinase in living cells are currently lacking. this paper describes the design, synthesis and characterization of f2, a high sensitive type of "turn-on" fluorescent probe for imaging living melanoma cells. f2 could be activated by tyrosinase-catalyzed oxidation followed by hydrolysis of a urea linkage. the results demonstrate that f2 displays cyan fluorescence when activated by tyrosinase, and has sufficient sensitivity and selectivity to detect tyrosinase in aqueous solution and in living cells. f2 has potential for the noninvasive real-time diagnosis and tracking of melanoma. (c) 2016 elsevier b.v. all rights reserved. |
| WOS关键词 | CONTINUOUS SPECTROPHOTOMETRIC METHOD ; DIPHENOLASE ACTIVITIES ; POLYPHENOL OXIDASE ; SKIN-CANCER ; MONOPHENOLASE ; NANOCLUSTERS ; INHIBITORS ; DOPAMINE ; PRODRUGS ; DOTS |
| WOS研究方向 | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
| WOS类目 | Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation |
| 语种 | 英语 |
| WOS记录号 | WOS:000393267700024 |
| 出版者 | ELSEVIER SCIENCE SA |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2176838 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Hu, Liming; Liang, Xing-Jie |
| 作者单位 | 1.Beijing Univ Technol, Coll Life Sci & Bioengn, Beijing 100124, Peoples R China 2.Chinese Acad Sci, CAS Ctr Excellence Nanosci, Beijing 100190, Peoples R China 3.Natl Ctr Nanosci & Technol China, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Tsinghua Univ, MOE Key Lab Bioinformat, Bioinformat Div, Beijing, Peoples R China 6.Tsinghua Univ, Ctr Synthet & Syst Biol, TNLIST, Beijing, Peoples R China 7.Tsinghua Univ, Dept Automat, Beijing, Peoples R China 8.Tsinghua Univ, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Minist Educ, Dept Chem, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Zhipeng,Wang, Yi-Feng,Zhang, Xu,et al. A tyrosinase-triggered oxidative reaction-based "turn-on" fluorescent probe for imaging in living melanoma cells[J]. Sensors and actuators b-chemical,2017,242:189-194. |
| APA | Li, Zhipeng,Wang, Yi-Feng,Zhang, Xu,Zeng, Chengchu,Hu, Liming,&Liang, Xing-Jie.(2017).A tyrosinase-triggered oxidative reaction-based "turn-on" fluorescent probe for imaging in living melanoma cells.Sensors and actuators b-chemical,242,189-194. |
| MLA | Li, Zhipeng,et al."A tyrosinase-triggered oxidative reaction-based "turn-on" fluorescent probe for imaging in living melanoma cells".Sensors and actuators b-chemical 242(2017):189-194. |
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