Study on the antigenicity of metallofullerenol: antibody production, characterization, and its enzyme immunoassay application
文献类型：期刊论文
| 作者 | Guo, Xihong; Yang, Shangyuan; Huang, Huan; Cui, Rongli; Li, Cheng; Yao, Huanli; Liu, Bing; Zhang, Lele; Xu, Binggang; Dong, Jinquan |
| 刊名 | Analytical and bioanalytical chemistry
 |
| 出版日期 | 2017-11-01 |
| 卷号 | 409期号:28页码:6575-6581 |
| 关键词 | Metallofullerene Enzyme immunoassay Polyclonal antibody Immunogen Nanomedicine |
| ISSN号 | 1618-2642 |
| DOI | 10.1007/s00216-017-0606-1 |
| 通讯作者 | Sun, baoyun(sunby@ihep.ac.cn) |
| 英文摘要 | With their intriguing structures and properties, metallofullerenols have attracted considerable attention in biological and medical applications. due to the increasing biomedical interest, effective detection methods are important to monitor and control metallofullerenols. however, the detection of metallofullerenols becomes very difficult after polyhydroxylated modification due to the lack of detectable features. antibody-based immunoassay methods have been important tools for detection and will better meet the needs of analysis of metallofullerenols. thus, the antigenicity of metallofullerenol has been studied for the first time. in this study, no immune response was detected when metallofullerenol gd@c-82(oh)(x) was used as immunogen. however, the polyclonal antibody against metallofullerenol was produced using metallofullerenol-klh (keyhole limpet hemocyanin) as immunogen, indicating that metallofullerenol can act as hapten. the specificity of the obtained antibody was investigated. it has been found that the hydroxyl groups on the surface of the carbon cage, the encapsulated metal, and the size and shape of the carbon cage did not affect the recognition specificity of the antibody. based on the obtained antibody, an indirect competitive enzyme immunoassay was developed for the determination of metallofullerenol with detection limits of 18 ng/ml in pbs. this enzyme immunoassay method was successfully used to detect metallofullerenol in serum. this work can provide an innovative way to determine metallofullerenols. |
| WOS关键词 | MRI CONTRAST AGENTS ; MONOCLONAL-ANTIBODIES ; NANOPARTICLES ; FULLERENES ; RELAXIVITY ; WATER ; C-60 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000414329900006 |
| 出版者 | SPRINGER HEIDELBERG |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2176875 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Sun, Baoyun |
| 作者单位 | Chinese Acad Sci, Inst High Energy Phys, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Xihong,Yang, Shangyuan,Huang, Huan,et al. Study on the antigenicity of metallofullerenol: antibody production, characterization, and its enzyme immunoassay application[J]. Analytical and bioanalytical chemistry,2017,409(28):6575-6581. |
| APA | Guo, Xihong.,Yang, Shangyuan.,Huang, Huan.,Cui, Rongli.,Li, Cheng.,...&Sun, Baoyun.(2017).Study on the antigenicity of metallofullerenol: antibody production, characterization, and its enzyme immunoassay application.Analytical and bioanalytical chemistry,409(28),6575-6581. |
| MLA | Guo, Xihong,et al."Study on the antigenicity of metallofullerenol: antibody production, characterization, and its enzyme immunoassay application".Analytical and bioanalytical chemistry 409.28(2017):6575-6581. |
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