Colloidal gold nanoparticles induce changes in cellular and subcellular morphology
文献类型：期刊论文
| 作者 | Ma, Xiaowei1,4; Hartmann, Raimo1; Jimenez de Aberasturi, Dorleta1,5; Yang, Fang2,3; Soenen, Stefaan J. H.6; Manshian, Bella B.6; Franz, Jonas7; Valdeperez, Daniel1; Pelaz, Beatriz1; Feliu, Neus1,8,9 |
| 刊名 | Acs nano
 |
| 出版日期 | 2017-08-01 |
| 卷号 | 11期号:8页码:7807-7820 |
| ISSN号 | 1936-0851 |
| 关键词 | Nanoparticles Au nps Cellular morphology Cytotoxicity Viability Cellular response |
| DOI | 10.1021/acsnano.7b01760 |
| 通讯作者 | Liang, xing-jie(liangxj@nanoctr.cn) ; Parak, wolfgang j.(wolfgang.parak@physik.uni-marburg.de) |
| 英文摘要 | Exposure of cells to colloidal nanoparticles (nps) can have concentration-dependent harmful effects. mostly, such effects are monitored with biochemical assays or probes from molecular biology, i.e., viability assays, gene expression profiles, etc., neglecting that the presence of nps can also drastically affect cellular morphology. in the case of polymer-coated au nps, we demonstrate that upon np internalization, cells undergo lysosomal swelling, alterations in mitochondrial morphology, disturbances in actin and tubulin cytoskeleton and associated signaling, and reduction of focal adhesion contact area and number of filopodia. appropriate imaging and data treatment techniques allow for quantitative analyses of these concentration-dependent changes. abnormalities in morphology occur at similar (or even lower) np concentrations as the onset of reduced cellular viability. cellular morphology is thus an important quantitative indicator to verify harmful effects of nps to cells, without requiring biochemical assays, but relying on appropriate staining and imaging techniques. |
| WOS关键词 | POLYMER-COATED NANOPARTICLES ; SURFACE FUNCTIONALITY ; SILVER NANOPARTICLES ; AMPHIPHILIC POLYMER ; TITANIUM-DIOXIDE ; MAMMALIAN-CELLS ; UNIVERSAL TOOL ; IN-VIVO ; CYTOTOXICITY ; TOXICITY |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Materials Science |
| WOS类目 | Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary |
| 语种 | 英语 |
| 出版者 | AMER CHEMICAL SOC |
| WOS记录号 | WOS:000408520900030 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2177034 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Liang, Xing-Jie; Parak, Wolfgang J. |
| 作者单位 | 1.Philipps Univ Marburg, Fachbereich Phys, D-35037 Marburg, Germany 2.Philipps Univ Marburg, Fachbereich Chem, D-35037 Marburg, Germany 3.Philipps Univ Marburg, Mat Sci Ctr, D-35037 Marburg, Germany 4.Chinese Acad Sci, Key Lab Biol Effects Nanomat & Nanosafety, Natl Ctr Nanosci & Technol, Beijing 100190, Peoples R China 5.CIC biomaGUNE, San Sebastian 20009, Spain 6.Katholieke Univ Leuven, Biomed MRI Unit MoSAIC, B-3000 Leuven, Belgium 7.Univ Munster, nAnost Inst, Ctr Nanotechnol, D-48149 Munster, Germany 8.Karolinska Inst, Dept Lab Med LABMED, SE-17177 Stockholm, Sweden 9.Medcom Adv SA, Barcelona 08840, Spain 10.Serend Ip GmbH, Ctr Nanotechnol, D-48149 Munster, Germany |
| 推荐引用方式 GB/T 7714 | Ma, Xiaowei,Hartmann, Raimo,Jimenez de Aberasturi, Dorleta,et al. Colloidal gold nanoparticles induce changes in cellular and subcellular morphology[J]. Acs nano,2017,11(8):7807-7820. |
| APA | Ma, Xiaowei.,Hartmann, Raimo.,Jimenez de Aberasturi, Dorleta.,Yang, Fang.,Soenen, Stefaan J. H..,...&Parak, Wolfgang J..(2017).Colloidal gold nanoparticles induce changes in cellular and subcellular morphology.Acs nano,11(8),7807-7820. |
| MLA | Ma, Xiaowei,et al."Colloidal gold nanoparticles induce changes in cellular and subcellular morphology".Acs nano 11.8(2017):7807-7820. |
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来源：高能物理研究所
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