Development of dynamic synchrotron radiation circular dichroism spectroscopy using continuous-flow probe with microfluidic mixer
文献类型：期刊论文
| 作者 | Wang Hao1,2; Huang Yang2; Liu Zhi-tong2; Gao Zhen-hua2; Sun Da-rui2; Tao Ye2 |
| 刊名 | Spectroscopy and spectral analysis
 |
| 出版日期 | 2017-07-01 |
| 卷号 | 37期号:7页码:2110-2114 |
| 关键词 | Microfluidic mixing Continuous flow Synchrotron radiation circular dichroism Protein folding |
| ISSN号 | 1000-0593 |
| DOI | 10.3964/j.issn.1000-0593(2017)07-2110-05 |
| 通讯作者 | Tao ye(taoy@ihep.ac.cn) |
| 英文摘要 | The setup of dynamic synchrotron radiation dichroism spectroscopy (srcd) is discussed by using continuous flow probe with microfluidic mixer, developed at 4b8 ultraviolet vacuum spectroscopy beamline at beijing synchrotron radiation facility. the quartz microfluidic mixer was fabricated with deep ion etching with channel depth down to 44. 5 microns. the mixer is designed on serpentine configuration to reach the effective mixing. the mixing efficiency was evaluated based on fluorescence image of the mixing solution under practical high viscous solution condition. dynamic srcd measurement with mixer was validated after implementing focus position feedback, since wavelength dispersion of the focusing lens results in the changes of focus spot position during wavelength scan. the time range covers from 4. 5 to 270 ms at present under 500 (l . min' flow rate within the wide observation channel. the performance of the,time-resolved srcd is demonstrated in snapshoting the folding of cytochrome c back to 54% at 4. 5 millisecond after completely mixing. |
| WOS研究方向 | Spectroscopy |
| WOS类目 | Spectroscopy |
| 语种 | 英语 |
| WOS记录号 | WOS:000405648400023 |
| 出版者 | OFFICE SPECTROSCOPY & SPECTRAL ANALYSIS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2177247 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Tao Ye |
| 作者单位 | 1.Anhui Univ, Inst Phys & Mat Sci, Mat Sci & Energy, Hefei 230601, Peoples R China 2.Chinese Acad Sci, BSRF, IHEP, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang Hao,Huang Yang,Liu Zhi-tong,et al. Development of dynamic synchrotron radiation circular dichroism spectroscopy using continuous-flow probe with microfluidic mixer[J]. Spectroscopy and spectral analysis,2017,37(7):2110-2114. |
| APA | Wang Hao,Huang Yang,Liu Zhi-tong,Gao Zhen-hua,Sun Da-rui,&Tao Ye.(2017).Development of dynamic synchrotron radiation circular dichroism spectroscopy using continuous-flow probe with microfluidic mixer.Spectroscopy and spectral analysis,37(7),2110-2114. |
| MLA | Wang Hao,et al."Development of dynamic synchrotron radiation circular dichroism spectroscopy using continuous-flow probe with microfluidic mixer".Spectroscopy and spectral analysis 37.7(2017):2110-2114. |
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来源：高能物理研究所
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