Detection of hepatitis b virus m204i mutation by quantum dot-labeled dna probe
文献类型：期刊论文
| 作者 | Zhang, Cheng1; Chen, Yiping2,3; Liang, Xinmiao4; Zhang, Guanhua1; Ma, Hong1; Nie, Leng5; Wang, Yu1 |
| 刊名 | Sensors
 |
| 出版日期 | 2017-05-01 |
| 卷号 | 17期号:5页码:9 |
| 关键词 | Quantum dot Hbv dna Sequencing Mutation |
| ISSN号 | 1424-8220 |
| DOI | 10.3390/s17050961 |
| 通讯作者 | Wang, yu(wangyuliver@ccmu.edu.cn) |
| 英文摘要 | Quantum dots (qds) are semiconductor nanoparticles with a diameter of less than 10 nm, which have been widely used as fluorescent probes in biochemical analysis and vivo imaging because of their excellent optical properties. sensitive and convenient detection of hepatitis b virus (hbv) gene mutations is important in clinical diagnosis. therefore, we developed a sensitive, low-cost and convenient qds-mediated fluorescent method for the detection of hbv gene mutations in real serum samples from chronic hepatitis b (chb) patients who had received lamivudine or telbivudine antiviral therapy. we also evaluated the efficiency of this method for the detection of drug-resistant mutations compared with direct sequencing. in chb, hbv dna from the serum samples of patients with poor response or virological breakthrough can be hybridized to probes containing the m204i mutation to visualize fluorescence under fluorescence microscopy, where fluorescence intensity is related to the virus load, in our method. at present, the limits of the method used to detect hbv genetic variations by fluorescence quantum dots is 103 iu/ml. these results show that qds can be used as fluorescent probes to detect viral hbv dna polymerase gene variation, and is a simple readout system without complex and expensive instruments, which provides an attractive platform for the detection of hbv m204i mutation. |
| WOS关键词 | REAL-TIME PCR ; DRUG-RESISTANCE ; HBV ; MANAGEMENT ; RECOMMENDATIONS ; LAMIVUDINE ; ASSAYS |
| WOS研究方向 | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
| WOS类目 | Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation |
| 语种 | 英语 |
| WOS记录号 | WOS:000404553300022 |
| 出版者 | MDPI AG |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2177269 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Wang, Yu |
| 作者单位 | 1.Capital Med Univ, Natl Clin Res Ctr Digest Dis, Liver Res Ctr, Beijing Friendship Hosp, 95 Yong An Rd, Beijing 100050, Peoples R China 2.Beijing Engn Res Ctr BioNanotechnol, Beijing 100190, Peoples R China 3.Chinese Acad Sci, Key Lab Biol Effects Nanomat & Nanosafety, Natl Ctr NanoSci & Technol, Beijing 100190, Peoples R China 4.Beijing Entry Exit Inspect & Quarantine Bur, Tianzhu Airport Ind Area, 3 Yi, Beijing 101312, Peoples R China 5.Wuhan Wawasye Nanotech Co, 36 Xinjiangongmen Rd, Beijing 100191, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Cheng,Chen, Yiping,Liang, Xinmiao,et al. Detection of hepatitis b virus m204i mutation by quantum dot-labeled dna probe[J]. Sensors,2017,17(5):9. |
| APA | Zhang, Cheng.,Chen, Yiping.,Liang, Xinmiao.,Zhang, Guanhua.,Ma, Hong.,...&Wang, Yu.(2017).Detection of hepatitis b virus m204i mutation by quantum dot-labeled dna probe.Sensors,17(5),9. |
| MLA | Zhang, Cheng,et al."Detection of hepatitis b virus m204i mutation by quantum dot-labeled dna probe".Sensors 17.5(2017):9. |
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来源：高能物理研究所
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