Probing temperature- and ph-dependent binding between quantum dots and bovine serum albumin by fluorescence correlation spectroscopy
文献类型：期刊论文
| 作者 | Wang, Zonghua1; Zhao, Qiyan1,2,3; Cui, Menghua2,4; Pang, Shichao5; Wang, Jingfang6; Liu, Ying3; Xie, Liming2,4 |
| 刊名 | Nanomaterials
 |
| 出版日期 | 2017-05-01 |
| 卷号 | 7期号:5页码:13 |
| 关键词 | Luminescent quantum dots Fluorescence correlation spectroscopy Temperature-and ph-dependent interactions Simulation |
| ISSN号 | 2079-4991 |
| DOI | 10.3390/nano7050093 |
| 通讯作者 | Wang, zonghua(wangzonghua@qdu.edu.cn) ; Liu, ying(liuy1@nanoctr.cn) ; Xie, liming(xielm@nanoctr.cn) |
| 英文摘要 | Luminescent quantum dots (qds) with unique optical properties have potential applications in bio-imaging. the interaction between qds and bio-molecules is important to the biological effect of qds in vivo. in this paper, we have employed fluorescence correlation spectroscopy (fcs) to probe the temperature- and ph-dependent interactions between cdse qds with carboxyl (qds-cooh) and bovine serum albumin (bsa) in buffer solutions. the results have shown that microscopic dissociation constant k'(d) is in the range of (1.5 +/- 0.2) x 10(-5) to (8.6 +/- 0.1) x 10(-7) m, the hill coefficient n is from 0.4 to 2.3, and the protein corona thickness is from 3.0 to 9.4 nm. variable-temperature measurements have shown both negative values of delta h and delta s for bsa adsorption on qds-cooh, while ph has a profound effect on the adsorption. additional, fcs measurement qds-cooh and proteins in whole mice serum and plasma samples has also been conducted. finally, simulation results have shown four favored qd binding sites in bsa. |
| WOS关键词 | ELECTROSTATIC INTERACTIONS ; SURFACE MODIFICATION ; GOLD NANOPARTICLES ; ENERGY-TRANSFER ; PROTEIN CORONA ; CARBOXYL GROUP ; FATTY-ACIDS ; IN-VITRO ; FLUOROPHORES ; NANOCRYSTALS |
| WOS研究方向 | Science & Technology - Other Topics ; Materials Science |
| WOS类目 | Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary |
| 语种 | 英语 |
| WOS记录号 | WOS:000404048600001 |
| 出版者 | MDPI AG |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2177315 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Wang, Zonghua; Liu, Ying; Xie, Liming |
| 作者单位 | 1.Qingdao Univ, Lab Fiber Mat & Modern Text,Growing Base State Ke, Collaborat Innovat Ctr Marine Biomass Fiber Mat &, Coll Chem & Chem Engn,Shandong Sino Japanese Ctr, Qingdao 266071, Peoples R China 2.Natl Ctr Nanosci & Technol, CAS Ctr Excellence Nanosci, CAS Key Lab Standardizat & Measurement Nanotechno, Beijing 100190, Peoples R China 3.Natl Ctr Nanosci & Technol, Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Shanghai 200240, Peoples R China 6.Shanghai Jiao Tong Univ, Shanghai Ctr Syst Biomed, Shanghai 200240, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Zonghua,Zhao, Qiyan,Cui, Menghua,et al. Probing temperature- and ph-dependent binding between quantum dots and bovine serum albumin by fluorescence correlation spectroscopy[J]. Nanomaterials,2017,7(5):13. |
| APA | Wang, Zonghua.,Zhao, Qiyan.,Cui, Menghua.,Pang, Shichao.,Wang, Jingfang.,...&Xie, Liming.(2017).Probing temperature- and ph-dependent binding between quantum dots and bovine serum albumin by fluorescence correlation spectroscopy.Nanomaterials,7(5),13. |
| MLA | Wang, Zonghua,et al."Probing temperature- and ph-dependent binding between quantum dots and bovine serum albumin by fluorescence correlation spectroscopy".Nanomaterials 7.5(2017):13. |
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来源：高能物理研究所
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