An automated and portable microfluidic chemiluminescence immunoassay for quantitative detection of biomarkers
文献类型：期刊论文
| 作者 | Hu, Binfeng1,2; Li, Juanjuan1; Mou, Lei1,2; Liu, Yong1,2; Deng, Jinqi1,2; Qian, Wei1,2; Sun, Jiashu1,2; Cha, Ruitao1; Jiang, Xingyu1,2 |
| 刊名 | Lab on a chip
 |
| 出版日期 | 2017-07-07 |
| 卷号 | 17期号:13页码:2225-2234 |
| ISSN号 | 1473-0197 |
| DOI | 10.1039/c7lc00249a |
| 通讯作者 | Sun, jiashu(sunjs@nanoctr.cn) ; Cha, ruitao(chart@nanoctr.cn) ; Jiang, xingyu(xingyujiang@nanoctr.cn) |
| 英文摘要 | Microfluidic platforms capable of automated, rapid, sensitive, and quantitative detection of biomarkers from patient samples could make a major impact on clinical or point-of-care (poc) diagnosis. in this work, we realize an automated diagnostic platform composed of two main components: (1) a disposable, self-contained, and integrated microfluidic chip and (2) a portable instrument that carries out completely automated operations. to demonstrate its potential for real-world application, we use injection molding for mass fabrication of the main components of disposable microfluidic chips. the assembled three-layered chip with on-chip mechanical valves for fluid control consists of (1) a top silicone fluidic layer with embedded zigzag microchannels, reagent reservoirs and a negative pressure port, (2) a middle tinfoil layer with patterned antibody/antigen stripes, and (3) a bottom silicone substrate layer with waste reservoirs. the versatility of the microfluidics-based system is demonstrated by implementation of a chemiluminescence immunoassay for quantitative detection of c-reactive protein (crp) and testosterone in real clinical samples. this lab-on-a-chip platform with features of quantitation, portability and automation provides a promising strategy for poc diagnosis. |
| WOS关键词 | C-REACTIVE PROTEIN ; CIRCULATING TUMOR-CELLS ; CARE ; PLATFORM ; SYSTEM ; CHIPS ; AMPLIFICATION ; MULTIPLEX ; LAB ; PATHOGENS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics |
| WOS类目 | Biochemical Research Methods ; Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology |
| 语种 | 英语 |
| WOS记录号 | WOS:000404469800008 |
| 出版者 | ROYAL SOC CHEMISTRY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2177340 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Sun, Jiashu; Cha, Ruitao; Jiang, Xingyu |
| 作者单位 | 1.Natl Ctr Nanosci & Technol, CAS Ctr Excellence Nanosci, CAS Key Lab Standardizat & Measurement Nanotechno, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Binfeng,Li, Juanjuan,Mou, Lei,et al. An automated and portable microfluidic chemiluminescence immunoassay for quantitative detection of biomarkers[J]. Lab on a chip,2017,17(13):2225-2234. |
| APA | Hu, Binfeng.,Li, Juanjuan.,Mou, Lei.,Liu, Yong.,Deng, Jinqi.,...&Jiang, Xingyu.(2017).An automated and portable microfluidic chemiluminescence immunoassay for quantitative detection of biomarkers.Lab on a chip,17(13),2225-2234. |
| MLA | Hu, Binfeng,et al."An automated and portable microfluidic chemiluminescence immunoassay for quantitative detection of biomarkers".Lab on a chip 17.13(2017):2225-2234. |
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来源：高能物理研究所
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