Integrative approach for the analysis of the proteome-wide response to bismuth drugs in helicobacter pylori
文献类型：期刊论文
| 作者 | Wang, Yuchuan1,2; Hu, Ligang1; Xu, Feng3; Quan, Quan1; Lai, Yau-Tsz1; Xia, Wei2; Yang, Ya1; Chang, Yuen-Yan1; Yang, Xinming1; Chai, Zhifang4 |
| 刊名 | Chemical science
 |
| 出版日期 | 2017-06-01 |
| 卷号 | 8期号:6页码:4626-4633 |
| ISSN号 | 2041-6520 |
| DOI | 10.1039/c7sc00766c |
| 通讯作者 | Sun, hongzhe(hsun@hku.hk) |
| 英文摘要 | Bismuth drugs, despite being clinically used for decades, surprisingly remain in use and effective for the treatment of helicobacter pylori infection, even for resistant strains when co-administrated with antibiotics. however, the molecular mechanisms underlying the clinically sustained susceptibility of h. pylori to bismuth drugs remain elusive. herein, we report that integration of in-house metalloproteomics and quantitative proteomics allows comprehensive uncovering of the bismuth-associated proteomes, including 63 bismuth-binding and 119 bismuth-regulated proteins from helicobacter pylori, with over 60% being annotated with catalytic functions. through bioinformatics analysis in combination with bioassays, we demonstrated that bismuth drugs disrupted multiple essential pathways in the pathogen, including ros defence and ph buffering, by binding and functional perturbation of a number of key enzymes. moreover, we discovered that hpdnak may serve as a new target of bismuth drugs to inhibit bacterium-host cell adhesion. the integrative approach we report, herein, provides a novel strategy to unveil the molecular mechanisms of antimicrobial metals against pathogens in general. this study sheds light on the design of new types of antimicrobial agents with multiple targets to tackle the current crisis of antimicrobial resistance. |
| WOS关键词 | METAL AFFINITY-CHROMATOGRAPHY ; BINDING PROTEINS ; MASS-SPECTROMETRY ; CELLS ; IDENTIFICATION ; RESISTANCE ; NETWORKS ; METALLOPROTEOMICS ; SUSCEPTIBILITY ; ERADICATION |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Multidisciplinary |
| 语种 | 英语 |
| WOS记录号 | WOS:000402384900056 |
| 出版者 | ROYAL SOC CHEMISTRY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2177385 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Sun, Hongzhe |
| 作者单位 | 1.Univ Hong Kong, Dept Chem, Pokfulam Rd, Hong Kong, Hong Kong, Peoples R China 2.Sun Yat Sen Univ, Sch Chem, Guangzhou, Guangdong, Peoples R China 3.Univ Hong Kong, Ctr Genome Sci, Hong Kong, Hong Kong, Peoples R China 4.Chinese Acad Sci, Inst High Energy Phys, CAS Key Lab Nucl Analyt Tech, Beijing, Peoples R China 5.Mayo Clin, Dept Hlth Sci Res, Ctr Individualized Med, Scottsdale, AZ 85259 USA 6.Arizona State Univ, Dept Biomed Informat, Scottsdale, AZ 85259 USA |
| 推荐引用方式 GB/T 7714 | Wang, Yuchuan,Hu, Ligang,Xu, Feng,et al. Integrative approach for the analysis of the proteome-wide response to bismuth drugs in helicobacter pylori[J]. Chemical science,2017,8(6):4626-4633. |
| APA | Wang, Yuchuan.,Hu, Ligang.,Xu, Feng.,Quan, Quan.,Lai, Yau-Tsz.,...&Sun, Hongzhe.(2017).Integrative approach for the analysis of the proteome-wide response to bismuth drugs in helicobacter pylori.Chemical science,8(6),4626-4633. |
| MLA | Wang, Yuchuan,et al."Integrative approach for the analysis of the proteome-wide response to bismuth drugs in helicobacter pylori".Chemical science 8.6(2017):4626-4633. |
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