Roles of leu28 side chain intercalation in the interaction between cren7 and dna
文献类型：期刊论文
| 作者 | Zhang, Zhenfeng1; Zhao, Mohan2; Wang, Li2; Chen, Yuanyuan3; Dong, Yuhui2; Gong, Yong2; Huang, Li1,4 |
| 刊名 | Biochemical journal
 |
| 出版日期 | 2017-05-15 |
| 卷号 | 474期号:10页码:1727-1739 |
| ISSN号 | 0264-6021 |
| DOI | 10.1042/bcj20170036 |
| 通讯作者 | Gong, yong(yonggong@ihep.ac.cn) ; Huang, li(huangl@sun.im.ac.cn) |
| 英文摘要 | Crenarchaeal chromatin protein cren7 binds double-stranded dna in the minor groove, introducing a sharp single-step dna kink. the side chain of leu28, a residue conserved among all cren7 homologs, intercalates into the kinked dna step. in the present study, we replaced leu28 with a residue containing a hydrophobic side chain of different sizes (i.e. l28a, l28v, l28i, l28m and l28f). both the stability of the cren7-dna complex and the ability of cren7 to constrain dna supercoils correlated well with the size of the intercalated side chain. structural analysis shows that l28a induces a kink (similar to 43 degrees), nearly as sharp as that produced by wild-type cren7 (similar to 48 degrees), in the bound dna fragment despite the lack of side chain intercalation. in another duplex dna fragment, l28f inserts a large hydrophobic side chain deep into the dna step, but introduces a smaller kink (similar to 39 degrees) than that formed by the wild-type protein (similar to 50 degrees). mutation of leu28 into methionine yields two protein conformers differing in loop beta 3-beta 4 orientation, dna-binding surface and dna geometry in the protein-dna structure. our results indicate that side chain intercalation is not directly responsible for dna kinking or bending by cren7, but plays a critical role in the stabilization of the cren7-dna complex. in addition, the flexibility of loop beta 3-beta 4 in cren7, as revealed in the crystal structure of l28m-dna, may serve a role in the modulation of chromosomal organization and function in the cell. |
| WOS关键词 | CHROMOSOMAL-PROTEIN SAC7D ; CRYSTAL-STRUCTURE ; ARCHAEAL CHROMATIN ; BINDING PROTEINS ; TERNARY COMPLEX ; TRANSCRIPTION ; ORGANIZATION ; COMPACTION ; SOFTWARE ; INSIGHTS |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000402145500011 |
| 出版者 | PORTLAND PRESS LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2177413 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Gong, Yong; Huang, Li |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, 1 West Beichen Rd, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst High Energy Phys, Beijing Synchrotron Radiat Facil, 19B YuquanLu, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Biophys, Nat Key Lab Biomacromol, 15 Datun Rd, Beijing 100101, Peoples R China 4.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Zhenfeng,Zhao, Mohan,Wang, Li,et al. Roles of leu28 side chain intercalation in the interaction between cren7 and dna[J]. Biochemical journal,2017,474(10):1727-1739. |
| APA | Zhang, Zhenfeng.,Zhao, Mohan.,Wang, Li.,Chen, Yuanyuan.,Dong, Yuhui.,...&Huang, Li.(2017).Roles of leu28 side chain intercalation in the interaction between cren7 and dna.Biochemical journal,474(10),1727-1739. |
| MLA | Zhang, Zhenfeng,et al."Roles of leu28 side chain intercalation in the interaction between cren7 and dna".Biochemical journal 474.10(2017):1727-1739. |
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来源：高能物理研究所
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