Effects of fullerenol nanoparticles on rat oocyte meiosis resumption
文献类型：期刊论文
| 作者 | Lei, Runhong1; Bai, Xue1; Chang, Yanan1; Li, Juan1; Qin, Yanxia1; Chen, Kui1; Gu, Weihong1; Xia, Shibo1; Zhang, Jiaxin1; Wang, Zhenbo2 |
| 刊名 | International journal of molecular sciences
 |
| 出版日期 | 2018-03-01 |
| 卷号 | 19期号:3页码:15 |
| 关键词 | Fullerenol Oocyte meiosis resumption Gap junction Granulosa cells Egfr/erk1/2 |
| ISSN号 | 1422-0067 |
| DOI | 10.3390/ijms19030699 |
| 通讯作者 | Wang, zhenbo(wangzb@ioz.ac.cn) ; Xing, gengmei(xinggm@ihep.ac.cn) |
| 英文摘要 | The excellent biocompatibility and biological effects of fullerenol and its derivatives make their biomedical application promising. the potential effects of fullerenol in mammals have been extensively studied, but little is known about its effects on female reproduction. using canonical oocyte-granulosa cell complexes (ogcs) in vitro maturation culture model, we investigated the effect of fullerenol on the first oocyte meiotic resumption. in the surrounding granulosa cells, fullerenol nanoparticles occluded the extracellular domain of the epidermal growth factor receptor (egfr) to reduce egfr-ligand binding and subsequent extracellular signal-regulated kinase 1 and 2 (erk1/2) activation, which involved the regulation of connexin 43 (cx43) expression and internalization. downregulation of cx43 expression and the retraction of transzonal projections (tzps) interrupted the gap junction channel and tzps based mass transportation. this effect decreased cyclic adenosine monophosphate (camp) levels in the oocyte and thereby accelerated rat oocyte meiosis resumption. moreover, perinuclear distribution of cx43 and egfr was observed in granulosa cells, which could further exacerbate the effects. fullerenol nanoparticles interfered with the strict process of oocyte meiosis resumption, which likely reduced the oocyte quality. |
| WOS关键词 | TITANIUM-DIOXIDE NANOPARTICLES ; SILVER ALLOY NANOPARTICLES ; GAP-JUNCTION ENDOCYTOSIS ; REPRODUCTIVE TOXICITY ; MAMMALIAN OOCYTE ; DEVELOPMENTAL COMPETENCE ; MEIOTIC MATURATION ; GRANULOSA-CELLS ; GRAPHENE OXIDE ; GROWTH-FACTORS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary |
| 语种 | 英语 |
| WOS记录号 | WOS:000428309800056 |
| 出版者 | MDPI |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2177515 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Wang, Zhenbo; Xing, Gengmei |
| 作者单位 | 1.Chinese Acad Sci, Inst High Energy Phys, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Zool, State Key Lab Stem Cell & Reprod Biol, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lei, Runhong,Bai, Xue,Chang, Yanan,et al. Effects of fullerenol nanoparticles on rat oocyte meiosis resumption[J]. International journal of molecular sciences,2018,19(3):15. |
| APA | Lei, Runhong.,Bai, Xue.,Chang, Yanan.,Li, Juan.,Qin, Yanxia.,...&Xing, Gengmei.(2018).Effects of fullerenol nanoparticles on rat oocyte meiosis resumption.International journal of molecular sciences,19(3),15. |
| MLA | Lei, Runhong,et al."Effects of fullerenol nanoparticles on rat oocyte meiosis resumption".International journal of molecular sciences 19.3(2018):15. |
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来源：高能物理研究所
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