An on-chip valve-assisted microfluidic chip for quantitative and multiplexed detection of biomarkers
文献类型：期刊论文
| 作者 | Hu, Binfeng1,2; Liu, Yong1,2; Deng, Jinqi1,2; Mou, Lei1,2; Jiang, Xingyu1,2 |
| 刊名 | Analytical methods
 |
| 出版日期 | 2018-06-07 |
| 卷号 | 10期号:21页码:2470-2480 |
| ISSN号 | 1759-9660 |
| DOI | 10.1039/c8ay00682b |
| 通讯作者 | Jiang, xingyu(xingyujiang@nanoctr.cn) |
| 英文摘要 | In this study, we developed a microfluidic chip integrated with on-chip valves for quantitative detection of biomarkers. the sandwich-structured microfluidic chip consists of a top fluidic layer embedded with a zigzag channel, a middle tinfoil layer pre-patterned with protein stripes, and a bottom substrate layer with two waste chambers. a set of customized on-chip valves was introduced for precise control of fluid flow. to improve the production volume and uniformity among chips, we selected an injection molding method for mass-production of the main components. we standardized the process of sealing chip layers and assembling the valve holder to improve the effectiveness of cooperation with the instrument. to demonstrate the versatility of the microfluidic chip for quantitative detection of biomarkers, we used it to automatically detect c-reactive proteins (crp), interleukin-6 (il-6), insulin, and osteocalcin with high sensitivity and excellent reproducibility inside a customized and portable instrument. we further conducted the automated chip-based chemiluminescence immunoassay to test crp in serum samples for demonstration of potential clinical applications, assess the activity of capture antibodies of crp (crp-ab(1)) on the tinfoil layer for preliminary assessment of storage stability, and simultaneously detect crp and il-6 for confirmation of scalable multiplexation. this microfluidic chip for quantitative, automated, and portable immunoassay provides a promising choice for clinical practices. |
| WOS关键词 | CHEMILUMINESCENCE IMMUNOASSAY ; PERFORMANCE EVALUATION ; AMPLIFICATION ; DEVICES ; CELLS ; QUANTIFICATION ; MICROVALVES ; EMBRYOS ; VIRUSES ; PUMPS |
| WOS研究方向 | Chemistry ; Food Science & Technology ; Spectroscopy |
| WOS类目 | Chemistry, Analytical ; Food Science & Technology ; Spectroscopy |
| 语种 | 英语 |
| WOS记录号 | WOS:000434311500007 |
| 出版者 | ROYAL SOC CHEMISTRY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2177536 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Jiang, Xingyu |
| 作者单位 | 1.Natl Ctr Nanosci & Technol, Beijing Engn Res Ctr BioNanotechnol, CAS Ctr Excellence Nanosci, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Binfeng,Liu, Yong,Deng, Jinqi,et al. An on-chip valve-assisted microfluidic chip for quantitative and multiplexed detection of biomarkers[J]. Analytical methods,2018,10(21):2470-2480. |
| APA | Hu, Binfeng,Liu, Yong,Deng, Jinqi,Mou, Lei,&Jiang, Xingyu.(2018).An on-chip valve-assisted microfluidic chip for quantitative and multiplexed detection of biomarkers.Analytical methods,10(21),2470-2480. |
| MLA | Hu, Binfeng,et al."An on-chip valve-assisted microfluidic chip for quantitative and multiplexed detection of biomarkers".Analytical methods 10.21(2018):2470-2480. |
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来源：高能物理研究所
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