Assessment of the mt1-mmp expression level of different cell lines by the naked eye
文献类型：期刊论文
| 作者 | Zhang, Xiangchun1,3; Yuan, Qing1,2,3; Gao, Xueyun1,2 |
| 刊名 | Science china-life sciences
 |
| 出版日期 | 2018-04-01 |
| 卷号 | 61期号:4页码:492-500 |
| 关键词 | Au clusters Mt1-mmp Catalyze Tumor cells |
| ISSN号 | 1674-7305 |
| DOI | 10.1007/s11427-017-9261-9 |
| 通讯作者 | Gao, xueyun(gaoxy@ihep.ac.cn) |
| 英文摘要 | Membrane type 1 matrix metalloproteinase (mt1-mmp) is expressed in most tumors and is believed to play a key role in the development, invasion and metastasis of tumors. there is an urgent need to develop a simple method to detect the mt1-mmp expression level on cells. in this current study, we demonstrated a red emission au cluster probes with the specific targeting of mt1-mmp on human dopaminergic neuroblastoma (sh-sy5y) cells. more importantly, utilizing the intrinsic enzyme-like activity of the au cluster probes, the expression level of mt1-mmp on the sh-sy5y cells could be assessed by the naked eye without cell lysis and protein extraction process. furthermore, sh-sy5y, human breast cancer (mcf-7), and human bronchial epithelial (16-hbe) cell lines with different mt1-mmp expression level could be distinguished using the au cluster probes by the naked eye. meanwhile, fluorescence intensity and au count determined by inductively coupled plasma mass spectrometry (icp-ms) were used to verify the feasibility of this simple analytical method. our proposed method is rapid, convenient, and accurate and could be assessed by the naked eye. this visual assessment method of tumor-associated proteins has immense implications in sorting tumor cells. |
| WOS关键词 | MATRIX METALLOPROTEINASES ; CANCER-CELLS ; MEMBRANE ; PROBE ; MICROENVIRONMENT ; CARCINOMA ; MARKING ; NUCLEI ; ENZYME |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics |
| WOS类目 | Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000431976200014 |
| 出版者 | SCIENCE PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2177591 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Gao, Xueyun |
| 作者单位 | 1.Chinese Acad Sci, Inst High Energy Phys, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100049, Peoples R China 2.Beijing Univ Technol, Dept Chem & Chem Engn, Beijing 100124, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Xiangchun,Yuan, Qing,Gao, Xueyun. Assessment of the mt1-mmp expression level of different cell lines by the naked eye[J]. Science china-life sciences,2018,61(4):492-500. |
| APA | Zhang, Xiangchun,Yuan, Qing,&Gao, Xueyun.(2018).Assessment of the mt1-mmp expression level of different cell lines by the naked eye.Science china-life sciences,61(4),492-500. |
| MLA | Zhang, Xiangchun,et al."Assessment of the mt1-mmp expression level of different cell lines by the naked eye".Science china-life sciences 61.4(2018):492-500. |
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来源：高能物理研究所
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