Directional mechanical stability of bacteriophage phi 29 motor's 3wj-prna: extraordinary robustness along portal axis
文献类型：期刊论文
| 作者 | Xu, Zhonghe1,2,3; Sun, Yang4; Weber, Jeffrey K.5; Cao, Yi4; Wang, Wei4; Jasinski, Daniel6,7,8; Guo, Peixuan6,7,8; Zhou, Ruhong1,2,5,9; Li, Jingyuan1,2,3 |
| 刊名 | Science advances
 |
| 出版日期 | 2017-05-01 |
| 卷号 | 3期号:5页码:8 |
| ISSN号 | 2375-2548 |
| DOI | 10.1126/sciadv.1601684 |
| 通讯作者 | Zhou, ruhong(ruhongz@us.ibm.com) ; Li, jingyuan(lijingyuan@ihep.ac.cn) |
| 英文摘要 | The molecular motor exploited by bacteriophage phi 29 to pack dna into its capsid is regarded as one of the most powerful mechanical devices present in viral, bacterial, and eukaryotic systems alike. acting as a linker element, a prohead rna (prna) effectively joins the connector and atpase (adenosine triphosphatase) components of the phi 29 motor. during dna packing, this prna needs to withstand enormous strain along the capsid's portal axis-how this remarkable stability is achieved remains to be elucidated. we investigate the mechanical properties of the phi 29 motor's junction (3wj)-prna using a combined steered molecular dynamics and atomic force approach. the 3wj exhibits strong resistance to stretching along its coaxial helices, demonstrating its super structural robustness. this resistance disappears, however, when external forces are applied to the transverse directions. from a molecular standpoint, we demonstrate that this direction-dependent stability can be attributed to clamps that cooperate and generate mechanical resistance in the prna's coaxial direction. our results suggest that the asymmetric nature of the 3wj's mechanical stability is entwined with its biological function: enhanced rigidity along the portal axis is likely essential to withstand the strain caused by dna condensation, and flexibility in other directions should aid in the assembly of the prna and its association with other motor components. |
| WOS关键词 | MOLECULAR-DYNAMICS SIMULATIONS ; DNA PACKAGING MOTOR ; PARTICLE MESH EWALD ; FORCE SPECTROSCOPY ; BIOMOLECULAR SIMULATIONS ; FUNCTIONAL DOMAINS ; INTERNAL FORCE ; 3-WAY JUNCTION ; VIRAL-RNA ; IN-VITRO |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000419752300003 |
| 出版者 | AMER ASSOC ADVANCEMENT SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2177905 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Zhou, Ruhong; Li, Jingyuan |
| 作者单位 | 1.Zhejiang Univ, Inst Quantitat Biol, Hangzhou 310027, Zhejiang, Peoples R China 2.Zhejiang Univ, Dept Phys, Hangzhou 310027, Zhejiang, Peoples R China 3.Chinese Acad Sci, Inst High Energy Phys, Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100049, Peoples R China 4.Nanjing Univ, Dept Phys, Natl Lab Solid State Microstruct, Collaborat Innovat Ctr Adv Microstruct, Nanjing 210093, Jiangsu, Peoples R China 5.IBM Thomas J Watson Res Ctr, Yorktown Hts, NY 10598 USA 6.Ohio State Univ, Coll Pharm, Div Pharmaceut & Pharmaceut Chem, Ctr RNA Nanobiotechnol & Nanomed, 500 W 12th Ave, Columbus, OH 43210 USA 7.Ohio State Univ, Coll Med, Dept Physiol & Cell Biol, Columbus, OH 43210 USA 8.Ohio State Univ, Dorothy M Davis Heart & Lung Res Inst, Columbus, OH 43210 USA 9.Columbia Univ, Dept Chem, New York, NY 10027 USA |
| 推荐引用方式 GB/T 7714 | Xu, Zhonghe,Sun, Yang,Weber, Jeffrey K.,et al. Directional mechanical stability of bacteriophage phi 29 motor's 3wj-prna: extraordinary robustness along portal axis[J]. Science advances,2017,3(5):8. |
| APA | Xu, Zhonghe.,Sun, Yang.,Weber, Jeffrey K..,Cao, Yi.,Wang, Wei.,...&Li, Jingyuan.(2017).Directional mechanical stability of bacteriophage phi 29 motor's 3wj-prna: extraordinary robustness along portal axis.Science advances,3(5),8. |
| MLA | Xu, Zhonghe,et al."Directional mechanical stability of bacteriophage phi 29 motor's 3wj-prna: extraordinary robustness along portal axis".Science advances 3.5(2017):8. |
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