Thermo-triggered release of crispr-cas9 system by lipid-encapsulated gold nanoparticles for tumor therapy
文献类型：期刊论文
| 作者 | Wang, Peng1; Zhang, Lingmin1,2,3; Zheng, Wenfu1; Cong, Liman4; Guo, Zhaorong4; Xie, Yangzhouyun1; Wang, Le1; Tang, Rongbing1; Feng, Qiang1; Hamada, Yoh4 |
| 刊名 | Angewandte chemie-international edition
 |
| 出版日期 | 2018-02-05 |
| 卷号 | 57期号:6页码:1491-1496 |
| 关键词 | Crispr/cas9 delivery Gold nanoparticles Lipids Photothermal effect Tumor therapy |
| ISSN号 | 1433-7851 |
| DOI | 10.1002/anie.201708689 |
| 通讯作者 | Zheng, wenfu(zhengwf@nanoctr.cn) ; Jiang, xingyu(xingyujiang@nanoctr.cn) |
| 英文摘要 | Crispr/cas9 system is a powerful toolbox for gene editing. however, the low delivery efficiency is still a big hurdle impeding its applications. herein, we report a strategy to deliver cas9-sgplk-1 plasmids (cp) by a multifunctional vehicle for tumor therapy. we condensed cps on tat peptide-modified au nanoparticles (aunps/cp, acp) via electrostatic interactions, and coated lipids (dotap, dope, cholesterol, peg2000-dspe) on the acp to form lipid-encapsulated, aunps-condensed cp (lacp). lacp can enter tumor cells and release cp into the cytosol by laser-triggered thermo-effects of the aunps; the cp can enter nuclei by tat guidance, enabling effective knock-outs of target gene (plk-1) of tumor (melanoma) and inhibition of the tumor both in vitro and in vivo. this aunps-condensed, lipid-encapsulated, and laser-controlled delivery system provides a versatile method for high efficiency crispr/cas9 delivery and targeted gene editing for treatment of a wide spectrum of diseases. |
| WOS关键词 | EFFICIENT DELIVERY ; GENOME ; DNA ; SIRNA ; CAS9 ; RNA |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Multidisciplinary |
| 语种 | 英语 |
| WOS记录号 | WOS:000423494500007 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2177932 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Zheng, Wenfu; Jiang, Xingyu |
| 作者单位 | 1.Natl Ctr NanoSci & Technol, CAS Ctr Excellence Nanosci, Beijing Engn Res Ctr BioNanotechnol, CAS Key Lab Biol Effects Nanomat & Nanosafety, 11 BeiYiTiao, Beijing 100190, Peoples R China 2.Guangzhou Med Univ, Sch Pharmaceut Sci, Guangdong Prov Key Lab Mol Target & Clin Pharmaco, Guangzhou 511436, Guangdong, Peoples R China 3.Guangzhou Med Univ, Affiliated Hosp 3 & 5, Guangzhou 511436, Guangdong, Peoples R China 4.Tohoku Univ, Grad Sch Med, Dept Nanomed Sci, Sendai, Miyagi 9808575, Japan 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Peng,Zhang, Lingmin,Zheng, Wenfu,et al. Thermo-triggered release of crispr-cas9 system by lipid-encapsulated gold nanoparticles for tumor therapy[J]. Angewandte chemie-international edition,2018,57(6):1491-1496. |
| APA | Wang, Peng.,Zhang, Lingmin.,Zheng, Wenfu.,Cong, Liman.,Guo, Zhaorong.,...&Jiang, Xingyu.(2018).Thermo-triggered release of crispr-cas9 system by lipid-encapsulated gold nanoparticles for tumor therapy.Angewandte chemie-international edition,57(6),1491-1496. |
| MLA | Wang, Peng,et al."Thermo-triggered release of crispr-cas9 system by lipid-encapsulated gold nanoparticles for tumor therapy".Angewandte chemie-international edition 57.6(2018):1491-1496. |
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