Device to study the cell invasion behavior and phenotypic profile at single cell level
文献类型：期刊论文
| 作者 | Li, Ren1,2,5; Ma, Yan1; Zhao, Minzhi1; Hu, Zhiyuan1,3,4; Wei, Zewen1 |
| 刊名 | Analytical chemistry
 |
| 出版日期 | 2018-02-06 |
| 卷号 | 90期号:3页码:1691-1700 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.7b03294 |
| 通讯作者 | Hu, zhiyuan(huzy@nanoctr.cn) ; Wei, zewen(weizw@nanoctr.cn) |
| 英文摘要 | Multiple methods for investigating cell invasion behavior in vitro have proven useful in exploring the mechanisms behind the epithelial-mesenchymal transition (emt) and emt-related tumor cell invasion, for example, by revealing that cell heterogeneity existed in emt. however, several hypotheses and predictions regarding emt heterogeneity have remained unproven because of the inability to quantitatively profile cell invasion at the single cell level. here, we present a microfluidic chip that provides the capability of simultaneously investigating single cell invasion behavior, phenotypic diversity, and responsiveness to anti invasion drugs. by assessing single cell invasion behavior in separate wells, cell cell contacts and their corresponding interference in the invasion process could be excluded. the chip allowed for both precise quantitation of cell invasion and in situ phenotyping, such that any single cell heterogeneity could be detected and accurately quantified. this study has proven that the proposed hybrid epithelial/mesenchymal cell phenotype exists and is important in the emt process. the invasion abilities of two cell lines were also assessed, either with or without emt-promoting or emt-inhibiting agents, proving that the chip can also be used to assess the effectiveness of antimetastatic agents. this study has demonstrated that the strategy of isolating single cells before studying their invasive properties is correct and that it provides an in vitro method for understanding cell heterogeneity during emt. this approach also provides a mean of screening for anti-invasion agents that are focused on single cell invasion, a process known to be important for blood-borne metastasis to occur. |
| 收录类别 | SCI |
| WOS关键词 | EPITHELIAL-MESENCHYMAL TRANSITION ; NEGATIVE BREAST-CANCER ; TUMOR-CELLS ; MIGRATION ; ASSAY ; EMT ; CYTOSKELETON ; NETWORKS ; MOTILITY |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000424730600038 |
| 出版者 | AMER CHEMICAL SOC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2177956 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Hu, Zhiyuan; Wei, Zewen |
| 作者单位 | 1.Natl Ctr Nanosci & Technol China, CAS Ctr Excellence Nanosci, CAS Key Lab Biomed Effects Nanomat & Nanosafety, CAS Key Lab Standardizat & Measurement Nanotechno, Beijing 100190, Peoples R China 2.Peking Univ, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China 3.Univ Chinese Acad Sci, Sinodanish Coll, Beijing 100049, Peoples R China 4.Yangtze River Delta Acad Nanotechnol & Ind Dev Re, Jiaxing 314000, Zhejiang, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Ren,Ma, Yan,Zhao, Minzhi,et al. Device to study the cell invasion behavior and phenotypic profile at single cell level[J]. Analytical chemistry,2018,90(3):1691-1700. |
| APA | Li, Ren,Ma, Yan,Zhao, Minzhi,Hu, Zhiyuan,&Wei, Zewen.(2018).Device to study the cell invasion behavior and phenotypic profile at single cell level.Analytical chemistry,90(3),1691-1700. |
| MLA | Li, Ren,et al."Device to study the cell invasion behavior and phenotypic profile at single cell level".Analytical chemistry 90.3(2018):1691-1700. |
入库方式： iSwitch采集
来源：高能物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。