Generation of a monoclonal antibody recognizing the heavily glycosylated cd45 protein and its application on identifying circulating tumor cells
文献类型：期刊论文
| 作者 | Zhang, Weikai1,2,3; Li, Zhitao1; Wang, Zihua3; Yue, Chunyan3; Zheng, Hui3; Li, Ren3; Zhou, Mingxing3; Hu, Zhiyuan3,4,5; Wei, Zewen3; Li, Qin2 |
| 刊名 | Plos one
 |
| 出版日期 | 2018-02-09 |
| 卷号 | 13期号:2页码:15 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0192506 |
| 通讯作者 | Hu, zhiyuan(huzy@nanoctr.cn) ; Wei, zewen(weizw@nanoctr.cn) ; Li, qin(liqin@bit.edu.cn) |
| 英文摘要 | Here, we provide direct evidence that using recombinant proteins expressed in eukaryotic cells as antigen is a practical way to generate monoclonal antibodies (mabs) against heavily glycosylated proteins. heavily glycosylated proteins are typically difficult targets for mab generation, being limited by unsatisfactory affinity and low specificity. using the heavily glycosylated cd45 protein as an example, we demonstrate the entire process of expressing the protein in eukaryotic cells and using it as an antigen to generate cd45-targeting mabs in mice. the mabs generated showed robust affinity and specificity, which are crucial factors for differentiate circulating tumor cells from white blood cells in human breast cancer patient samples. only 1 cell fusion and 2 cyclic sub-cloning steps were necessary before mabs with satisfactory performance were obtained. |
| WOS关键词 | CANCER-PATIENTS ; IDENTIFICATION ; MARKER ; BLOOD ; ELISA |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000424700800026 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2177977 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Hu, Zhiyuan; Wei, Zewen; Li, Qin |
| 作者单位 | 1.Henan Univ Sci & Technol, Med Coll, Luoyang, Peoples R China 2.Beijing Inst Technol, Sch Life Sci, Dept Biomed Engn, Beijing, Peoples R China 3.Natl Ctr Nanosci & Technol China, CAS Ctr Excellence Nanosci, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing, Peoples R China 4.Univ Chinese Acad Sci, Sinodanish Coll, Beijing, Peoples R China 5.Yangtze River Delta Acad Nanotechnol & Ind Dev Re, Jiaxing, Zhejiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Weikai,Li, Zhitao,Wang, Zihua,et al. Generation of a monoclonal antibody recognizing the heavily glycosylated cd45 protein and its application on identifying circulating tumor cells[J]. Plos one,2018,13(2):15. |
| APA | Zhang, Weikai.,Li, Zhitao.,Wang, Zihua.,Yue, Chunyan.,Zheng, Hui.,...&Li, Qin.(2018).Generation of a monoclonal antibody recognizing the heavily glycosylated cd45 protein and its application on identifying circulating tumor cells.Plos one,13(2),15. |
| MLA | Zhang, Weikai,et al."Generation of a monoclonal antibody recognizing the heavily glycosylated cd45 protein and its application on identifying circulating tumor cells".Plos one 13.2(2018):15. |
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来源：高能物理研究所
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