Enzyme-free amplification strategy for biosensing using fe3+-poly(glutamic acid) coordination chemistry
文献类型：期刊论文
| 作者 | Wu, Jing1; Xianyu, Yunlei3; Wang, Xiangfeng1; Hu, Dehua1; Zhao, Zhitao1; Lu, Ning2; Xie, Mengxia1; Lei, Hongtao2; Chen, Yiping3 |
| 刊名 | Analytical chemistry
 |
| 出版日期 | 2018-04-03 |
| 卷号 | 90期号:7页码:4725-4732 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.7b05344 |
| 通讯作者 | Xie, mengxia(xiemx@bnu.edu.cn) ; Lei, hongtao(hongtao@scau.edu.cn) ; Chen, yiping(cyp89@126.com) |
| 英文摘要 | In this work, we outline a signal amplification strategy using the coordination chemistry between fe(3+)and poly(glutamic acid) (pga) for biosensing applications. the theoretical calculation based on density functional theory shows that pga has a much higher binding affinity with fe3+ than the other metal ions. guided by this rationale, we prepare a pga-mediated signal probe through conjugating pga onto polystyrene (ps) nanoparticles to form a brushlike nanostructure for fe3+ coordination. this pga-ps brush (ppb) has a large loading capacity of fe3+ with a number of 1.92 x 10(8) fe atoms per nanoparticle that greatly amplifies the signals for assays in an enzyme-free way. combined with ferrozine coloration-based readout, this ppb-mediated amplification is further applied for the enzyme-free immunoassay that shows an ultrahigh sensitivity for detection of microcystins-lr (12 pg/ml), a 5-fold enhancement compared with that of traditional enzyme-linked immunosorbent assay (elisa) (60 pg/ml). in addition, the good stability, rapid response, and long shelf life make this enzyme-free amplification strategy a promising platform for point-of-care biosensing applications. |
| WOS关键词 | BIOPOLYMER POLY(GAMMA-GLUTAMIC ACID) ; ULTRASENSITIVE DETECTION ; COVALENT IMMOBILIZATION ; SIGNAL AMPLIFICATION ; DISEASE BIOMARKERS ; COLORIMETRIC ASSAY ; HEAVY-METALS ; BRUSHES ; ELISA ; IMMUNOSENSOR |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| 语种 | 英语 |
| 出版者 | AMER CHEMICAL SOC |
| WOS记录号 | WOS:000429385800067 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2178193 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Xie, Mengxia; Lei, Hongtao; Chen, Yiping |
| 作者单位 | 1.Beijing Normal Univ, Analyt & Testing Ctr, Beijing 100875, Peoples R China 2.South China Agr Univ, Coll Food Sci, Guangdong Prov Key Lab Food Qual & Safety, Guangzhou 510642, Guangdong, Peoples R China 3.Natl Ctr Nanosci & Technol, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Jing,Xianyu, Yunlei,Wang, Xiangfeng,et al. Enzyme-free amplification strategy for biosensing using fe3+-poly(glutamic acid) coordination chemistry[J]. Analytical chemistry,2018,90(7):4725-4732. |
| APA | Wu, Jing.,Xianyu, Yunlei.,Wang, Xiangfeng.,Hu, Dehua.,Zhao, Zhitao.,...&Chen, Yiping.(2018).Enzyme-free amplification strategy for biosensing using fe3+-poly(glutamic acid) coordination chemistry.Analytical chemistry,90(7),4725-4732. |
| MLA | Wu, Jing,et al."Enzyme-free amplification strategy for biosensing using fe3+-poly(glutamic acid) coordination chemistry".Analytical chemistry 90.7(2018):4725-4732. |
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来源：高能物理研究所
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