Through-bond energy transfer cassette with dual-stokes shifts for "double checked" cell imaging
文献类型：期刊论文
| 作者 | Xue, Xiangdong1,2,3; Jin, Shubin1,2,3,4; Li, Zhipeng5; Zhang, Chunqiu1,2; Guo, Weisheng1,2,3; Hu, Liming5; Wang, Paul C.6,7; Zhang, Jinchao8; Liang, Xing-Jie1,2,3 |
| 刊名 | Advanced science
 |
| 出版日期 | 2017-12-01 |
| 卷号 | 4期号:12页码:7 |
| ISSN号 | 2198-3844 |
| 关键词 | Cell imaging Energy transfer Fluorescence Pseudo-stokes shift |
| DOI | 10.1002/advs.201700229 |
| 通讯作者 | Liang, xing-jie(liangxj@nanoctr.cn) |
| 英文摘要 | Organic dyes generally suffer from small stokes shift that usually leads to self-quenching and -gaining errors during the fluorescent imaging process. here, a through-bond energy transfer (tbet) cassette is developed with large stokes shift to pursue precise cell imaging. the tbet is constructed by covalently conjugated tetraphenylethene (acts as donor) and rhodamine (acceptor) through an acetylene bond. the constructed tbet cassette distinctly behaves as dual-stokes shifts, including a large pseudo-stokes shift caused by energy transfer, from donor's absorption to acceptor's emission (up to 260 nm) and a smaller stokes shift of acceptor molecules itself. due to the intrinsic dual-stokes shifts, tbet cassette exhibits specific dual distinct absorbances, single shared emission properties, which can be excitated under two different laser channels. by colocalization of the imaging readouts of these two channels, the precisely double checked fluorescent imaging is achieved in living cells. |
| WOS关键词 | AGGREGATION-INDUCED EMISSION ; LIVING CELLS ; IN-VIVO ; FLUORESCENCE ; PROBE ; FRET ; DYES ; NANOPARTICLES ; DELIVERY ; CANCER |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Materials Science |
| WOS类目 | Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary |
| 语种 | 英语 |
| 出版者 | WILEY |
| WOS记录号 | WOS:000418385800001 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2178259 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Liang, Xing-Jie |
| 作者单位 | 1.Chinese Acad Sci, Ctr Excellence Nanosci, 11 Beiyitiao, Beijing 100190, Peoples R China 2.Chinese Acad Sci, CAS Key Lab Biol Effects Nanomat & Nanosafety, Natl Ctr Nanosci & Technol, 11 Beiyitiao, Beijing 100190, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Beijing Municipal Inst Labor Protect, Beijing 100054, Peoples R China 5.Beijing Univ Technol, Sch Life Sci, Beijing 100124, Peoples R China 6.Fu Jen Catholic Univ, Coll Sci & Engn, Taipei 24205, Taiwan 7.Howard Univ, Dept Radiol, Lab Mol Imaging, Washington, DC 20060 USA 8.Hebei Univ, Coll Chem & Environm Sci, Baoding 071002, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xue, Xiangdong,Jin, Shubin,Li, Zhipeng,et al. Through-bond energy transfer cassette with dual-stokes shifts for "double checked" cell imaging[J]. Advanced science,2017,4(12):7. |
| APA | Xue, Xiangdong.,Jin, Shubin.,Li, Zhipeng.,Zhang, Chunqiu.,Guo, Weisheng.,...&Liang, Xing-Jie.(2017).Through-bond energy transfer cassette with dual-stokes shifts for "double checked" cell imaging.Advanced science,4(12),7. |
| MLA | Xue, Xiangdong,et al."Through-bond energy transfer cassette with dual-stokes shifts for "double checked" cell imaging".Advanced science 4.12(2017):7. |
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来源：高能物理研究所
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