An effective and low-cost method for DNA extraction from herbal drugs of Rheum tanguticum (polygonaceae)
文献类型：期刊论文
| 作者 | Hu, Yanping1,2; Xie, Xiaolong1,2; Wang, Li1; Yang, Jian1,2; Zhang, Huaigang1; Li, Yi1 |
| 刊名 | african journal of biotechnology
 |
| 出版日期 | 2009-06-17 |
| 卷号 | 8期号:12页码:2691-2694 |
| 关键词 | Rhubarb Rheum tanguticum DNA extraction ISSR secondary metabolites |
| ISSN号 | 1684-5315 |
| 中文摘要 | rhubarb is an important traditional chinese herbal drug with high secondary metabolites that interfere with dna extraction procedures and downstream applications, such as dna restriction and amplification. an effective and low-cost protocol for isolating genomic dna from root of rheum tanguticum is described in this paper. it involved a modified ctab method with distilled water pretreating samples. the a(260)/a(280) absorbance ratio of extracted dna, free from polysaccharide, polyphenols and tannins contaminants, ranged from 1.85 to 2.0 within the high level of purity. the dna preparations were found suitable for analyses with restriction enzyme digestion and inter-simple sequence repeats (issr). this method is helpful for further studies on rhubarb at molecular level and is more cost-effective than commercial kit. |
| 英文摘要 | rhubarb is an important traditional chinese herbal drug with high secondary metabolites that interfere with dna extraction procedures and downstream applications, such as dna restriction and amplification. an effective and low-cost protocol for isolating genomic dna from root of rheum tanguticum is described in this paper. it involved a modified ctab method with distilled water pretreating samples. the a(260)/a(280) absorbance ratio of extracted dna, free from polysaccharide, polyphenols and tannins contaminants, ranged from 1.85 to 2.0 within the high level of purity. the dna preparations were found suitable for analyses with restriction enzyme digestion and inter-simple sequence repeats (issr). this method is helpful for further studies on rhubarb at molecular level and is more cost-effective than commercial kit. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biotechnology & applied microbiology |
| 研究领域[WOS] | biotechnology & applied microbiology |
| 关键词[WOS] | conservation concern ; gene sequence ; pcr analysis ; identification ; optimization ; plants |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000268831200006 |
| 公开日期 | 2011-12-13 |
| 源URL | [http://ir.nwipb.ac.cn//handle/363003/1856]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Yanping,Xie, Xiaolong,Wang, Li,et al. An effective and low-cost method for DNA extraction from herbal drugs of Rheum tanguticum (polygonaceae)[J]. african journal of biotechnology,2009,8(12):2691-2694. |
| APA | Hu, Yanping,Xie, Xiaolong,Wang, Li,Yang, Jian,Zhang, Huaigang,&Li, Yi.(2009).An effective and low-cost method for DNA extraction from herbal drugs of Rheum tanguticum (polygonaceae).african journal of biotechnology,8(12),2691-2694. |
| MLA | Hu, Yanping,et al."An effective and low-cost method for DNA extraction from herbal drugs of Rheum tanguticum (polygonaceae)".african journal of biotechnology 8.12(2009):2691-2694. |
入库方式： OAI收割
来源：西北高原生物研究所
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